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US 8,557,534 B2Granted
Method and compositions for specifically detecting physiologically acceptable polymer molecules
AssigneesBaxter International Inc., Baxter Healthcare S.A.
InventorsPeter Turecek, Juergen Siekmann, Alfred Weber, Herbert Gritsch, Katalin Varadi, Susanne Vejda
Life of this patent
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Abstract
The present invention relates to a method for determining the amount of a physiologically acceptable polymer molecule bound to a protein, an antibody or other composition being capable of specifically binding to a physiologically acceptable polymer molecule, and a kit containing said antibody or composition.
Claims14
A method for determining the number of polyethylene glycol (PEG) polymer molecules in a polymer:protein conjugate having one or more PEG polymer molecules bound to the protein, the method comprising:
- contacting the polymer:protein conjugate with an antibody that specifically binds the PEG polymer of the polymer:protein conjugate, the antibody detectable when bound to the polymer:protein conjugate,
- detecting the level of the antibody bound to the polymer:protein conjugate,
- wherein the number of PEG polymer molecules in the polymer:protein conjugate correlates with the level of the antibody detected bound to the polymer:protein conjugate when compared to a known control, and wherein antibody binding is dose dependent and linear with respect to an amount of bound PEG.
Dependencies are inferred from the claim text.