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US 8,557,530 B2Granted
Use of a TAS2R7 nucleic acid sequence to identify modulators
AssigneeGivaudan SA
InventorsJay Patrick Slack, Jenny Ellen Evans Pennimpede
Life of this patent
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Abstract
TAS2R7 was identified as a menthol-binding bitter taste receptor. Novel methods to identify modulators and in particular inhibitors to the bitter taste of menthol are provided.
Claims22
A method to identify an agent that modulates the taste of menthol, the method comprising:
- (i) contacting cells that express a bitter taste receptor comprising a polypeptide sequence with a sequence identity of at least 90% to SEQ ID NO:2, which is able to be activated by menthol with menthol in the presence of at least one agent;
- and
- (ii) determining whether the at least one agent affects binding to menthol or a functional response of said bitter taste receptor to menthol,
- with the proviso that the cells are not unmanipulated cells in their natural environment.
1 more element
A kit comprising:
- (i) recombinant cells that express a bitter taste receptor comprising a polypeptide sequence with a sequence identity of at least 90% to SEQ ID NO:2, and
- (ii) menthol,
- for combined use to identify agents as modulators of the taste of menthol.
A method to identify an agent that modulates a bitter taste receptor comprising a polypeptide sequence with a sequence identity of at least 90% to SEQ ID NO:2, which is able to be activated by menthol, the method comprising the steps of:
- (i) measuring a parameter that changes in response to menthol binding to the bitter taste receptor, and
- (ii) determining a change of the parameter in response to an agent in presence of menthol, in comparison to a negative control comprising menthol but not the agent, and thereby identifying the agent as a modulator of the taste of menthol.
Dependencies are inferred from the claim text.