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US 8,557,524 B2Granted
Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
AssigneeMolecular Detection, Inc.
InventorYosef Paitan
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Abstract
The present invention relates generally to detection of antibiotic-resistant bacteria in a sample. In particular, the invention provides methods, compositions and kits for detecting and analyzing methicillin-resistant Staphylococcus aureus (MRSA) and other methicillin-resistant bacteria in a sample.
Claims15
A method of detecting methiceillin-resistant S. aureus (MRSA) in a sample, wherein said sample contains one or a mixture of bacterial species, said method comprising:
- (a) amplifying a mecA polynucleotide sequence to produce an amplified mecA polynucleotide sequence;
- (b) determining a cycle threshold (CT) value of said amplified mecA polynucleotide sequence;
- (c) amplifying a bridging region polynucleotide sequence from said sample to produce an amplified bridging region polynucleotide sequence;
- (d) determining a CT value of said amplified bridging region polynucleotide sequence;
- (e) amplifying an S. aureus-specific polynucleotide sequence to produce an amplified S. aureus-specific polynucleotide sequence;
- (f) determining a CT value of said amplified S. aureus-specific polynucleotide sequence, wherein said S. aureus-specific polynucleotide sequence is not an orfX polynucieotide sequence,
- (g) comparing said CT values of said amplified mecA, amplified bridging region, and amplified S. aureus-specific polynucleotide sequences to each other,
- wherein if said CT values of each of said amplified mecA polynueleotide, said amplified bridging region polynueleotide, and said amplified S. aureus-specific polynucleotide sequences are within a predetermined range to each other, then MRSA is present in said sample.
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Dependencies are inferred from the claim text.