Method of nucleic acid amplification and measuring reagent and reagent kit therefor

A method for nucleic acid amplification using an internal standard material, comprising:

• amplifying a target nucleic acid;
• and
• amplifying the internal standard material;
• wherein amplifying the target nucleic acid and amplifying the internal standard material occur under isothermal conditions in a same reaction solution,
• wherein the amplifying of the internal standard material is initiated prior to initiating the amplifying of the target nucleic acid, thereby reducing the influence of the amplified internal standard material on the amplifying of the target nucleic acid;
• wherein the internal standard material has a nucleotide sequence different from that of the target nucleic acid at least in the primer design region;
• wherein the oligonucleotide primer used for amplifying the internal standard material specifically hybridizes to the internal standard material but does not specifically hybridize to the target nucleic acid, and the oligonucleotide primer for amplifying the target nucleic acid specifically hybridizes to the target nucleic acid but does not specifically hybridize to the internal standard material;
• wherein the oligonucleotide primer for amplifying the internal standard material initiates amplifying the internal standard material earlier than the initiation of amplification of the target nucleic acid.