Methods for obtaining bioactive compounds from phytoplankton
AssigneesWoods Hole Oceanographic Institution, Rutgers, The State University of New Jersey
InventorsKay Daniel Bidle, Assaf Vardi, Benjamin A. S. Van Mooy, Helen F. Fredricks, Liti Haramaty
Life of this patent
Estimating the term…
Abstract
Phytoplankton represent a potential source of bioactive compounds. The present disclosure provides, inter alia, methods for identifying glycerolipids and apoptosis-inducing sphingosine-like lipids from virally-infected phytoplankton.
Claims18
A method for identifying one or more apoptosis-inducing glycosylated sphingolipids, comprising:
- (a) obtaining a sample of one or more glycosylated sphingolipids from a virally-infected phytoplankton;
- (b) contacting a target cell with the sample;
- (c) assaying the target cell for apoptosis-associated activity in the presence of the sample;
- wherein apoptosis-associated activity in the target cell indicates that the sample comprises one or more apoptosis-inducing glycosylated sphingolipids.
A method of manufacturing one or more glycosylated sphingolipids, comprising:
- (a) infecting phytoplankton with a virus;
- (b) culturing the infected phytoplankton;
- and
- (c) isolating the one or more glycosylated sphingolipids from the infected phytoplankton.
A method for identifying one or more glycerolipids capable of inhibiting viral-mediated apoptosis, comprising:
- (a) obtaining a sample of one or more glycerolipids from a virally-infected phytoplankton;
- (b) contacting a target cell with the sample;
- and
- (c) assaying the target cell for apoptosis-associated activity in the presence of the sample and further in the presence of a virus that induces apoptosis in the target cell in the absence of the sample;
- wherein a decrease in apoptosis-associated activity in the target cell relative to the apoptosis-associated activity in the absence of the sample indicates that the sample comprises one or more glycerolipids capable of inhibiting viral-mediated apoptosis.
Dependencies are inferred from the claim text.