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US 8,557,513 B2Granted
Methods for treating and/or limiting development of diabetes
AssigneeBioCrine AB
InventorsPer-Olof Berggren, Shao-Nian Yang
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for identifying candidate compounds for limiting development of and/or treating diabetes, and methods for limiting development of and/or treating diabetes.
Claims10
An in vitro method for identifying candidate compounds for limiting development of and/or treating diabetes, comprising
- (a) contacting a population of insulin secreting cells with an amount of apolipoprotein CIII (ApoCIII) effective to increase density and/or conductivity of Cav1 channels, in the presence of one or more test compounds;
- and
- (b) identifying those positive test compounds that inhibit an ApoCIII-induced increase in density and/or conductivity of Cav1 channels in the population of insulin secreting cells compared to control, wherein the control comprises contacting a control population of insulin secreting cells with an amount of ApoCIII effective to increase density and/or conductivity of Cav1 channels, in the presence of the one or more test compounds and further contacting the control population of insulin secreting cells with a Src kinase inhibitor and a protein kinase A (PKA) inhibitor, wherein positive test compounds that inhibit the ApoCIII-induced increase in density and/or conductivity of Cav1 channels in the population of insulin secreting cells to a greater degree than in the control population of insulin secreting cells are candidate compounds for limiting development of and/or treating diabetes.
Dependencies are inferred from the claim text.