Process for producing a microCT image of a stained specimen
AssigneeBoard of Regents, The University of Texas System
InventorsCharles Keller, Ali N. Bahadur
Life of this patent
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Abstract
Disclosed is a process of producing a microCT image of a stained specimen, wherein said specimen is a non-human organ, said process comprising: incubating a specimen in a first staining composition, said first staining composition comprising a first staining agent, to produce a stained specimen; submerging said stained specimen in a liquid with an electron density lower than that of said stained specimen; and placing said stained specimen in a specimen holder and scanning said stained specimen in an X-ray computed tomography scanner to produce said microCT image of said stained specimen.
Claims31
A process of producing a microCT image of a stained specimen, wherein said specimen is an organ said process comprising:
- (a) incubating a specimen in a first staining composition, said first staining composition comprising a first staining agent, to produce a stained specimen;
- (b) submerging said stained specimen in a liquid with an electron density lower than that of said stained specimen;
- and
- (c) placing said submerged, stained specimen in a specimen holder and scanning said submerged, stained specimen in an X-ray computed tomography scanner to produce said microCT image of said submerged, stained specimen.
A process of producing a microCT image of a stained specimen, wherein said specimen is a tissue said process comprising:
- (a) incubating a specimen in a first staining composition, said first staining composition comprising a first staining agent, to produce a stained specimen;
- (b) suspending said stained specimen in a liquid with an electron density lower than that of said stained specimen;
- (c) incubating the specimen in a second staining composition comprising a second staining agent, to produce a double-stained specimen;
- and
- (d) placing the specimen in a specimen holder and scanning said stained specimen in an X-ray computed tomography scanner to produce said microCT image of said suspended, double stained specimen.
Dependencies are inferred from the claim text.