Microscope having internal focusing

A control method for a laser scanning microscope with a scanner and a microscope objective, and further including at least one of (1) a tube lens which is adjustable along an optical axis of the microscope, (2) an optical element which is adjustable along the optical axis in the microscope objective, and (3) collimating optics which are displaceable along the optical axis, a focus position of the microscope being adjustable relative to a front optical element of the microscope objective by adjusting the tube lens, the optical element, or the collimating optics;

• wherein the following steps are carried out repeatedly:
• an optical section of a sample is scanned by means of the scanner;
• and
• the focus position is subsequently adjusted by adjusting the tube lens, the optical element, or the collimating optics;
• and
• wherein a movement of the scanner is influenced based on a given correction value for an imaging scale of the adjusted focus position in such a way that different imaging scales are compensated for during recording of optical sections and all of the optical sections of the sample are recorded with approximately the same imaging scale.