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US 8,551,968 B2Granted
Methods for generation of antibodies
AssigneeNational Jewish Health
InventorsYosef Refaeli, Brian Curtis Turner
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Abstract
This invention generally relates to methods for the production of antibody producing cells and antibodies in protooncogene expressing animals. The invention also relates to methods for the efficient production of antibodies specific for antigens that are normally subject to immunological constraints such as self tolerance. The invention further relates to the production of antibody producing cells and antibodies without the need for the conventional fusing of antibody producing B cells with a myeloma fusion partner.
Claims12
A method for producing an antibody to an antigen comprising:
- a) providing a non-human mammal that inducibly overexpresses MYC, wherein the non-human mammal was produced by
- transferring a mammalian hematopoietic stem cell into the non-human mammal, wherein the mammalian hematopoietic stem cell comprises a recombinant nucleic acid molecule encoding an inducible MYC and a recombinant nucleic acid molecule encoding an antigen, and wherein the non-human mammal was lethally-irradiated or sub lethally-irradiated prior to having the hematopoietic stem cell transferred into the non-human mammal;
- b) inducing the overexpression of MYC in the non-human-mammal after formation of mature B cells from the transferred mammalian hematopoietic stem cell;
- c) recovering a B cell overexpressing MYC from the non-human mammal, wherein the B cell comprises the recombinant nucleic acid molecule encoding the inducible MYC and the recombinant nucleic acid molecule encoding the antigen;
- d) culturing the B cell overexpressing MYC under conditions in which MYC overexpression is maintained;
- and
- e) recovering the antibody from the B cell culture.
4 more elements
Dependencies are inferred from the claim text.