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US 8,551,920 B2Granted
Libraries and methods for isolating antibodies
AssigneeMorpho Sys AG
InventorsRene Hoet, Sonia Schoonbroodt, Robert C. Ladner
Life of this patent
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Abstract
The disclosure features methods for identifying antibodies that bind to a carbohydrate moiety. Libraries coding for antibodies that bind to a carbohydrate moiety are provided. The libraries can be provided by modifying a pre-existing nucleic acid library. Antibodies that bind to a carbohydrate moiety are described.
Claims14
A method for identifying an antibody or fragment thereof that binds to a carbohydrate comprising:
- a) providing a protein library comprising a plurality of diverse antibodies or fragments thereof, wherein said antibodies or fragments thereof comprise a heavy chain variable domain sequence and a light chain variable domain sequence, wherein said heavy chain variable domain sequence comprises diversity in HC CDR3, and wherein each antibody or fragment thereof comprises an HC CDR3 comprising (G/S)-X-(K/R)-X-(K/R)-X (SEQ ID NO:3), wherein X is varied among a set of amino acids that exclude basic amino acids;
- b) contacting said protein library to a carbohydrate;
- and
- c) identifying one or more antibodies or fragments thereof that binds to said carbohydrate, wherein the protein library comprises at least 105 antibodies or fragments thereof.
1 more element
A method for providing an antibody coding library biased for carbohydrate binding, comprising:
- a) providing an antibody coding library that includes a plurality of members, wherein each member comprises a sequence encoding a heavy chain variable domain sequence, wherein the HC variable domain coding sequences are diverse among members of the plurality;
- b) providing nucleic acids that encode a diverse population of HC CDR3s, wherein said HC CDR3 coding sequences encode HC CDR3s comprising (G/S)-X-(K/R)-X-(K/R)-X (SEQ ID NO:3), wherein X is varied among a set of amino acids that exclude basic amino acids;
- and
- c) modifying the HC variable domain coding sequences of said antibody coding library to include the HC CDR3 coding sequences;
- and
- d) obtaining an antibody coding library biased for carbohydrate binding, wherein the antibody coding library comprises at least 105 nucleic acids.
3 more elements
Dependencies are inferred from the claim text.