Recombinant hosts and methods for manufacturing polyhydroxyalkanoate

A recombinant host for manufacturing polyhydroxyalkanoate, wherein

• native succinate semialdehyde dehydrogenase gene is removed from said recombinant host;
• and
• a first plasmid and a second plasmid including genes for manufacturing the polyhydroxyalkanoate are transformed to the recombinant host, the first plasmid includes 4-hydroxybutyrate-CoA:CoA transferase gene (orfZ gene), β-ketothiolase gene (phaA gene), NADPH-dependent acetoacetyl-CoA reductase gene (phaB gene), and polyhydroxyalkanoate synthase gene (phaC gene), and the second plasmid includes succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene);
• characterized in that the second plasmid further includes pyruvate decarboxylase promoter (Ppdc);
• wherein the first plasmid has the sequence of SEQ ID NO:
• 1 and the second plasmid has the sequence of SEQ ID NO:
• 7.