Method of producing non-bovine chymosin and use hereof
AssigneesEidgenossische Technische Hochschule Zurich, Chr. Hansen A/S
InventorsStefan Kappeler, Zakaria Farah, Johannes Maarten Van den Brink, Henrik Rahbek-Nielsen, Peter Budtz
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Abstract
A method of recombinantly producing a non-bovine pre-prochymosin, prochymosin or chymosin derived from ruminant species including deer species, buffalo species, antelope species, giraffe species, ovine species and caprine species; Camelidae species such as Camelus dromedarius; porcine species; or Equidae species. The recombinant enzymes are used in milk coagulating compositions in cheese manufacturing based on cow's milk and milk from any animal species which are used in cheese manufacturing including camel's milk.
Claims25
A method of manufacturing a non-tylopodal cheese, comprising adding, to a non-tylopodal milk, a milk clotting effective amount of a composition comprising a milk clotting agent that is a recombinant protein produced by expression of a nucleic acid sequence encoding the protein in a suitable heterologous host cell and comprising a tylopodal protein selected from the group consisting of tylopodal pre-prochymosin, tylopodal prochymosin and tylopodal chymosin, thereby clotting the milk to obtain a curd and a whey, and separating the curd from the whey to obtain said non-tylopodal cheese.
In a method of manufacturing a non-tylopodal cheese from a non-tylopodal milk, the improvement comprising clotting the milk by adding to the milk a milk clotting effective amount of a composition comprising a milk clotting agent that is a recombinant protein produced by expression of a nucleic acid sequence encoding the protein in a suitable heterologous host cell, and comprising a tylopodal protein selected from the group consisting of tylopodal pre-prochymosin, tylopodal prochymosin and tylopodal chymosin.
Dependencies are inferred from the claim text.