# US 8,543,182 B2 · Implantable biosensor and methods of use thereof

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- Number: US 8,543,182 B2
- Document ID: US8543182B2
- Status: Granted
- Kind: B2
- Application number: 11/427,641
- Filed: 2006-06-29
- Published: 2006-10-26
- Granted: 2013-09-24
- Earliest claimed: 2003-12-18
- Assignees: Metronom Health, Inc.
- Applicants: Elliot Botvinick; Troy M. Bremer
- Inventors: Elliot Botvinick; Troy M. Bremer
- Web page: https://patentgrep.com/patent/US8543182B2

## CPC

- A61B 5/1473: invasive, e.g. introduced into the body by a catheter
- A61B 5/14532: {for measuring glucose, e.g. by tissue impedance measurement}
- A61B 5/1455: using optical sensors, e.g. spectral photometrical oximeters
- A61B 5/1459: invasive, e.g. introduced into the body by a catheter
- A61B 5/14865: {invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors}
- A61B 5/1495: Calibrating or testing of in-vivo probes
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)

## Abstract

Provided herein is a stabilized oxygen transport matrix that includes a reversible oxygen binding protein, such as hemoglobin, immobilized throughout the stabilized oxygen transport matrix. The stabilized oxygen transport matrix is used to transport oxygen and can be used as an oxygen transport region and a reaction region of an analyte sensor, such as an implantable glucose sensor. The reversible binding protein can also function as an oxygen probe within the analyte sensor.

## Claims

### Claim 1

A device, comprising:
a) a first oxygen permeable surface;
b) a second oxygen permeable surface; and
c) a stabilized oxygen transport matrix in between the first and second oxygen permeable surfaces, wherein the stabilized oxygen transport matrix comprises a reversible oxygen binding protein immobilized throughout the stabilized oxygen transport matrix, and wherein the stabilized oxygen transport matrix transports oxygen from the first oxygen permeable surface to the second oxygen permeable surface.

### Claim 2 (depends on 1)

The device of claim 1, wherein the reversible oxygen binding protein is an engineered hemeprotein, or a heme derivative.

### Claim 3 (depends on 1)

The device of claim 1, wherein the reversible oxygen binding protein is hemoglobin.

### Claim 4 (depends on 1)

The device of claim 1, wherein the reversible oxygen binding protein is present at about 10% by mass or higher.

### Claim 5 (depends on 1)

The device of claim 1, further comprising a carrier protein.

### Claim 6 (depends on 5)

The device of claim 5, wherein the carrier protein is albumin, gelatin or a combination thereof.

### Claim 7 (depends on 5)

The device of claim 5, wherein the carrier protein is present at about 1 to 15% by weight.

### Claim 8 (depends on 1)

The device of claim 1, further comprising a polymer selected from the group consisting of polyglycolic acid, polylactic acid, poly (lactic-co-glycolic acid), poly(caprolactone), poly(dioxanone), polytetrafluoroethylene, expanded polytetrafluoroethylene, polypropylene, polyethylene glycol, and polyvinyl alcohol.

### Claim 9 (depends on 1)

The device of claim 1, further comprising a coating of silicon, rubber or polymer.

### Claim 10 (depends on 1)

The device of claim 1, further comprising a membrane.

### Claim 11 (depends on 10)

The device of claim 10, wherein the membrane is a non-natural membrane.

### Claim 12 (depends on 10)

The device of claim 10, wherein the membrane is made from a naturally derived material selected from the group consisting of collagen, alginate, agarose, hyaluronic acid derivatives, chitosan, and fibrin glue.

### Claim 13 (depends on 10)

The device of claim 10, wherein the membrane is made from a synthetic polymer selected from the group consisting of polyglycolic acid, polylactic acid, poly(lactic-co-glycolic acid), poly(caprolactone), poly(dioxanone), polytetrafluoroethylene, expanded polytetrafluoroethylene, polypropylene, polyethylene glycol, and polyvinyl alcohol.

### Claim 14 (depends on 1)

A biosensor, comprising the device of claim 1.

### Claim 15 (depends on 1)

The device of claim 1, wherein the partial pressure of oxygen at the first oxygen permeable surface is greater than the partial pressure of oxygen at the second oxygen permeable surface.