# US 8,542,357 B2 · Method and device for measuring circular dichroism spectra > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,542,357 B2 - Document ID: US8542357B2 - Status: Granted - Kind: B2 - Application number: 12/935,853 - Filed: 2009-04-03 - Published: 2011-03-17 - Granted: 2013-09-24 - Earliest claimed: 2008-04-04 - Assignees: The University of Tokyo - Applicants: Hiromi Takahashi; Reiko Kuroda; Takunori Harada - Inventors: Hiromi Takahashi; Reiko Kuroda; Takunori Harada - Web page: https://patentgrep.com/patent/US8542357B2 ## CPC - G01N 21/19: Dichroism - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract It is an object of the present invention to solve the above-mentioned problems by using an optical arrangement that is totally different from the conventional CD spectra measuring method, and to provide a method and device for measuring circular dichroism spectra, which can measure the CD spectrum in much shorter time even with the use of a small light source. The method for measuring circular dichroism spectra according to the present invention comprising the steps of irradiating a white light projected from a white light source to a sample without dispersing the light into a monochromatic light, dispersing the light output from the sample, detecting a light intensity of the dispersed light by a detector having a sensor consisting of a charge-coupled device, and then measuring a circular dichroism spectrum of the sample on the basis of a result of the detection by the detector (FIG. 1). ## Claims ### Claim 1 A method for measuring circular dichroism spectra comprising the steps of: irradiating a white light projected from a white light source to a sample without dispersing the light into a monochromatic light, dispersing a light output from the sample, detecting a light intensity of the dispersed light by a detector having a sensor consisting of a charge-coupled device, and then measuring a circular dichroism spectrum of the sample on the basis of a result of the detection by the detector, wherein a light input timing into the detector, which detector includes a micro-channel plate, is regulated by the micro-channel plate. ### Claim 2 (depends on 1) The method for measuring circular dichroism spectra according to claim 1, wherein: the light projected from the light source is linearly polarized by a light polarizer, the linearly polarized light is circularly polarized by a polarization modulator, and then the polarized light is irradiated to the sample. ### Claim 3 (depends on 2) The method of measuring circular dichroism spectra according to claim 2, wherein the regulating of the light input timing by the micro-channel plate is on the basis of a modulating frequency of the polarization modulator. ### Claim 4 (depends on 1) The method for measuring circular dichroism spectra according to claim 1, wherein: the light output from the sample is circularly polarized by a polarization modulator, the circularly polarized light is linearly polarized by a light polarizer, and then the linearly polarized light is dispersed by a spectroscope. ### Claim 5 (depends on 4) The method of measuring circular dichroism spectra according to claim 4, wherein the regulating of the light input timing by the micro-channel plate is on the basis of a modulating frequency of the polarization modulator. ### Claim 6 (depends on 1) The method for measuring circular dichroism spectra according to claim 1, wherein: the sample is disposed on a total reflection boundary surface, an evanescent light is produced when the light is reflected on the total reflection boundary surface and is used for measuring an optical absorption, and then circular dichroism spectrum is measured on the basis of a difference between a right-handed circularly polarized light element and a left-handed circularly polarized light element of the light. ### Claim 7 (depends on 6) The method for measuring circular dichroism spectra according to claim 6, wherein: the reflection surface on which the sample is disposed comprises a reflection surface of a sample cell that includes one of an optical wave-guide, a cylindrical shape prism, a hemisphere shape prism or a polygonal shape prism, the white light is irradiated to the sample cell, and the measuring of the circular dichroism spectrum is performed on the basis of the light that is reflected from the sample on the reflection surface of the sample cell. ### Claim 8 (depends on 1) The method of measuring circular dichroism spectra according to claim 1, wherein: a dependence of polarization degree of the circularly polarized light produced by a polarization modulator on a wavelength is reduced by a summation of block spectra on the respective wavelength of the light intensity that is detected by the sensor. ### Claim 9 A device for measuring circular dichroism spectra comprising: a white light source which irradiates a white light, a sample cell in which a sample is disposed, a spectroscope dispersing the light output from the sample cell, and a detector detecting a light intensity of the dispersed light by the spectroscope, which has a sensor consisting of a charge-coupled device, wherein the white light source, the sample cell, the spectroscope, and the detector are disposed on a light path of the white light in series, the white light is irradiated from the white light source to the sample without dispersing the light into a monochromatic light, the light output from the sample is dispersed, the light intensity of the dispersed light is detected by the detector having the sensor consisting of the charge-coupled device, and then a circular dichroism spectrum of the sample is measured on the basis of the light intensity of the dispersed light by the detector, and wherein said detector comprises a micro-channel plate, and wherein a light input timing into the detector is regulated by the micro-channel plate. ### Claim 10 (depends on 9) The device for measuring circular dichroism spectra according to claim 9, wherein: a light polarizer and a polarization modulator are disposed in series between the white light source and the sample cell. ### Claim 11 (depends on 9) The device for measuring circular dichroism spectra according to claim 9, wherein: a light polarizer and a polarization modulator are disposed in series between the sample cell and the spectroscope. ### Claim 12 (depends on 9) The device for measuring circular dichroism spectra according to claim 9, wherein: said sample cell has a total reflection boundary surface on which said sample is disposed. ### Claim 13 (depends on 12) The device for measuring circular dichroism spectra according to claim 12, wherein: said sample cell comprises any one of an optical wave-guide, a cylindrical shape prism, a hemisphere shape prism or a polygonal shape prism. ### Claim 14 A method for measuring circular dichroism spectra comprising the steps of: irradiating a white light projected from a white light source to a sample without dispersing the light into a monochromatic light, dispersing the light output from the sample, polarizing one of the white light or dispersed light both linearly by a light polarizer and circularly by a polarization modulator, detecting a light intensity of the dispersed light by a detector having a sensor consisting of a charge-coupled device, and then measuring a circular dichroism spectrum of the sample on the basis of a result of the detection by the detector, and wherein a dependence of polarization degree of the circularly polarized light on a wavelength is reduced by a summation of block spectra on the respective wavelength of the light intensity that is detected by the sensor. ### Claim 15 (depends on 14) The method for measuring circular dichroism spectra according to claim 14, wherein: the light projected from the light source is linearly polarized by the light polarizer, the linearly polarized light is circularly polarized by the polarization modulator, and then the polarized light is irradiated to the sample. ### Claim 16 (depends on 14) The method for measuring circular dichroism spectra according to claim 14, wherein: the light output from the sample is circularly polarized by the polarization modulator, the circularly polarized light is linearly polarized by the light polarizer, and then the linearly polarized light is dispersed by a spectroscope. ### Claim 17 (depends on 14) The method for measuring circular dichroism spectra according to claim 14, wherein: the sample is disposed on a total reflection boundary surface, an evanescent light is produced when the light is reflected on the total reflection boundary surface and is used for measuring an optical absorption, and then circular dichroism spectrum is measured on the basis of a difference between a right-handed circularly polarized light element and a left-handed circularly polarized light element of the light. ### Claim 18 (depends on 17) The method for measuring circular dichroism spectra according to claim 17, wherein: the reflection surface on which the sample is disposed comprises a reflection surface of a sample cell that includes one of an optical wave-guide, a cylindrical shape prism, a hemisphere shape prism or a polygonal shape prism, the white light is irradiated to the sample cell, and the measuring of the circular dichroism spectrum is performed on the basis of the light that is reflected from the sample on the reflection surface of the sample cell.