# US 8,541,735 B2 · Inlaid optical material and method of manufacture > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,541,735 B2 - Document ID: US8541735B2 - Status: Granted - Kind: B2 - Application number: 12/662,237 - Filed: 2010-04-07 - Published: 2011-10-13 - Granted: 2013-09-24 - Earliest claimed: 2010-04-07 - Assignees: Innovative Micro Technology - Applicants: John S. Foster; John C. Harley; Ian R. Johnston; Jeffery F. Summers - Inventors: John S. Foster; John C. Harley; Ian R. Johnston; Jeffery F. Summers - Web page: https://patentgrep.com/patent/US8541735B2 ## CPC - G01N 21/645: {Specially adapted constructive features of fluorimeters} - G01N 15/1459: {the analysis being performed on a sample stream} - G01N 15/1484: {microstructural devices} - G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)} - G01N 15/149: specially adapted for sorting particles, e.g. by their size or optical properties - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract An optical material is inlaid into a supporting substrate, with the top surface of the optical material flush with the top surface of the substrate, wherein the optical element is used to shape a beam of light travelling substantially parallel to the top surface of the substrate, but with the central axis of the beam below the top surface of the substrate. The optical elements serve to shape the beam of light for delivery to or from a microfabricated structure within the device. ## Claims ### Claim 1 A microdevice comprising: a substrate having a top surface; at least one void formed in the top surface; a first optical element comprising a first optically transmissive material having an index of refraction and contained in the at least one void, wherein a top surface of the optically transmissive material does not extend beyond the top surface of the substrate and wherein the first optically transmissive material comprises at least one of SiOz, glass, InTiO2, photoresist, and sapphire, and wherein the first optical element is configured to transmit a beam of light propagating in a plane substantially parallel to the top surface of the substrate and has a symmetry axis which is substantially parallel to the top surface; and a second optically transmissive material having a different index of refraction and disposed adjacent to the first optically transmissive material, and configured to either reflect or refract the beam of light at a boundary between the first and the second optically transmissive materials, and wherein the second optically transmissive material comprises at least one of air, water and an oil. ### Claim 2 (depends on 1) The microdevice of claim 1, wherein the first optically transmissive material is configured to focus the beam of light to a focal point adjacent to the first optically transmissive material. ### Claim 3 (depends on 2) The microdevice of claim 2, wherein the first and second optically transmissive materials are configured to shape the beam of light and deliver it to a target portion of the microdevice, which receives the beam shaped by the first and second optically transmissive materials. ### Claim 4 (depends on 2) The microdevice of claim 2, further comprising a second optical element comprising the first optically transmissive material contained in a second void formed in the substrate surface, wherein the first optically transmissive material is substantially flush with the top surface of the substrate, wherein the second optical element further shapes the beam of light transmitted through the first optical element. ### Claim 5 (depends on 4) The microdevice of claim 4, wherein the second optical material is substantially the same as the first optical material, and the first and second optical elements are lenses having different focal lengths. ### Claim 6 (depends on 3) The microdevice of claim 3, wherein the target portion of the microdevice is at least one of a microfabricated cell sorter, a cell counter, and optical emitter and an optical detector, and wherein a cover is affixed to the top surface of the substrate, to form at least one fluid channel in the substrate. ### Claim 7 (depends on 1) The microdevice of claim 1, wherein the shape of the first optical element is at least one of circular, parabolic, hyperbolic, dispersive, reflective and refractive. ### Claim 8 (depends on 1) The microdevice of claim 1, wherein the first optical element is configured to perform at least one of diffract, disperse, diffuse, reflect, refract or focus the beam of light. ### Claim 9 (depends on 5) The microdevice of claim 5, wherein the first optically transmissive material is silicon dioxide, the second optically transmissive material is open air, and the first optical element focuses the beam of light to a focal point within the second optically transmissive material, and wherein the first and second optical elements have a symmetry axis which is collinear with a line bisecting the first and second optical elements. ### Claim 10 (depends on 9) The microdevice of claim 9, wherein the first and second optical elements shape the beam of light into parallel rays directed to a detection region of a microfluidic channel. ### Claim 11 A method for making a microdevice, comprising: forming a first void in a top surface of a substrate; and depositing a thin layer of conductive material conformally over the top surface of the substrate and into the first void; forming a first optical element by depositing a first optically transmissive material having an index of refraction into the first void; planarizing the top surface of the first optically transmissive material until it is substantially flush with the top surface of the substrate, wherein the first optical element is configured to interact with a beam of light propagating in a plane substantially parallel to the top surface of the substrate; and forming a second void into the top surface of the substrate, adjacent to and contiguous with the first optical element. ### Claim 12 (depends on 11) The method of claim 11, further comprising: etching a third void in the top surface of the substrate, configured to transport a volume of fluid from an input reservoir to an output reservoir. ### Claim 13 (depends on 12) The method of claim 12, further comprising: etching a movable actuator into the top surface of the substrate, configured to separate one component of the fluid from other components of the fluid. ### Claim 14 (depends on 12) The method of claim 12, further comprising: bonding an optically transmissive second substrate to the top surface of the substrate and over the third void, to form microfluidic channels confining the fluid, but allowing light to pass through to and from the microfluidic channels. ### Claim 15 (depends on 14) The method of claim 14, wherein the substrate comprises a silicon-on-insulator substrate, the optically transmissive second substrate comprises a glass substrate, and the first optically transmissive material comprises silicon dioxide. ### Claim 16 (depends on 11) The method of claim 11, wherein the first optical element is configured to perform at least one of diffract, disperse, diffuse, reflect, refract or focus the beam of light. ### Claim 17 (depends on 11) The method of claim 11, wherein the first optical element has a shape selected from circular, parabolic, and hyperbolic, and a functionality selected from at least one of dispersive, reflective, refractive, diffractive, prismatic, birefringent, and polarization rotating.