# US 8,541,665 B2 · Polynucleotides and polypeptides in plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,541,665 B2 - Document ID: US8541665B2 - Status: Granted - Kind: B2 - Application number: 12/917,303 - Filed: 2010-11-01 - Published: 2011-03-31 - Granted: 2013-09-24 - Earliest claimed: 2002-09-18 - Assignees: Mendel Biotechnology, Inc. - Applicants: Cai-Zhong Jiang; Jacqueline E. Heard; Oliver Ratcliffe; Robert A. Creelman; Luc Adam; T. Lynne Reuber; Jose Luis Riechmann; Volker Haake; Arnold N. Dubell; James S. Keddie; Bradley K. Sherman - Inventors: Cai-Zhong Jiang; Jacqueline E. Heard; Oliver Ratcliffe; Robert A. Creelman; Luc Adam; T. Lynne Reuber; Jose Luis Riechmann; Volker Haake; Arnold N. Dubell; James S. Keddie; Bradley K. Sherman - Web page: https://patentgrep.com/patent/US8541665B2 ## CPC - C07K 14/415: from plants - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed. ## Claims ### Claim 1 A transgenic plant that has an improved trait relative to a control plant, wherein the transgenic plant comprises an expression vector or cassette comprising a recombinant polynucleotide that encodes a polypeptide; wherein the polypeptide comprises a conserved domain that is at least 87% identical to amino acids 65-137 of SEQ ID NO: 594; wherein the polypeptide is at least 79% identical over the entire length of SEQ ID NO:594; wherein said control plant does not comprise the expression vector or cassette; and wherein over-expression of the polypeptide in the transgenic plant confers to the transgenic plant an improved trait selected from the group consisting of greater yield and greater tolerance to cold, relative to a control plant. ### Claim 2 (depends on 1) The transgenic plant of claim 1, wherein the polypeptide is at least 85% identical over the entire length of SEQ ID NO: 594. ### Claim 3 (depends on 1) The transgenic plant of claim 1, wherein the polypeptide comprises a conserved domain that is at least 95% identical to amino acids 65-137 of SEQ ID NO: 594. ### Claim 4 (depends on 1) The transgenic plant of claim 1, wherein the polypeptide is at least 95% identical over the entire length of SEQ ID NO: 594. ### Claim 5 (depends on 1) The transgenic plant of claim 1, wherein the polypeptide comprises SEQ ID NO: 594. ### Claim 6 (depends on 1) The transgenic plant of claim 1, wherein the transgenic plant is more tolerant to a six hour exposure to 4°-8° C. during its growth than the control plant. ### Claim 7 (depends on 1) The transgenic plant of claim 1, wherein the recombinant polynucleotide further comprises a constitutive, inducible, or tissue-specific promoter that regulates expression of the polypeptide. ### Claim 8 (depends on 1) The transgenic plant of claim 1, wherein the transgenic plant is a corn or maize plant. ### Claim 9 (depends on 1) A leaf, stem, flower, fruit or plant tissue of the transgenic plant of claim 1, said leaf, stem, flower, fruit or plant comprising said expression vector or cassette. ### Claim 10 (depends on 9) The plant tissue of claim 9, wherein said plant tissue is ground tissue. ### Claim 11 A method for producing a transgenic plant that has an improved trait relative to a control plant, the method steps comprising: (a) providing a recombinant construct encoding a polypeptide that comprises a conserved domain that is at least 87% identical to amino acids 65-137 of SEQ ID NO: 594; wherein the polypeptide is at least 79% identical over the entire length of SEQ ID NO: 594; and (b) introducing the recombinant construct into a target plant to produce a transgenic plant; wherein when the polypeptide is over-expressed in a plant, the polypeptide confers an improved trait that is selected from the group consisting of greater yield and greater tolerance to cold, relative to a control plant that does not comprise the recombinant construct. ### Claim 12 (depends on 11) The method of claim 11, wherein the polypeptide is at least 85% identical over the entire length of SEQ ID NO: 594. ### Claim 13 (depends on 11) The method of claim 11, wherein the polypeptide comprises a conserved domain that is at least 95% identical to amino acids 65-137 of SEQ ID NO: 594. ### Claim 14 (depends on 11) The method of claim 11, wherein the polypeptide is at least 95% identical over the entire length of SEQ ID NO: 594. ### Claim 15 (depends on 11) The method of claim 11, wherein the polypeptide comprises SEQ ID NO: 594. ### Claim 16 (depends on 11) The method of claim 11, wherein the method further comprises the step of: (c) identifying a transgenic plant by selecting a transgenic plant that overexpresses the polypeptide relative to the control plant. ### Claim 17 (depends on 11) The method of claim 11, wherein the recombinant construct further comprises a constitutive, inducible, or tissue-specific promoter that regulates expression of the polypeptide. ### Claim 18 (depends on 11) The method of claim 11, wherein the transgenic plant is a corn or maize plant. ### Claim 19 A transgenic corn or maize plant that has greater yield than a control corn or maize plant, wherein the transgenic corn or maize plant comprises an expression vector or cassette comprising a recombinant polynucleotide that encodes a polypeptide; wherein the polypeptide comprises a conserved domain that is at least 87% identical to amino acids 65-137 of SEQ ID NO: 594, and wherein the polypeptide is at least 79% identical to SEQ ID NO: 594; wherein said control corn or maize plant does not comprise the expression vector or cassette; and wherein overexpression of the polypeptide in the transgenic corn or maize plant confers to the transgenic corn or maize plant greater yield. ### Claim 20 (depends on 19) The transgenic corn or maize plant of claim 19, wherein the expression vector or cassette comprises a constitutive, inducible, or tissue-specific promoter that regulates expression of the polypeptide.