# US 8,541,654 B2 · Use of recombinant polypeptides from the genus Lupinus as antimicrobial or insecticidal on plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,541,654 B2 - Document ID: US8541654B2 - Status: Granted - Kind: B2 - Application number: 12/893,889 - Filed: 2010-09-29 - Published: 2011-04-14 - Granted: 2013-09-24 - Earliest claimed: 2005-07-21 - Assignees: Instituto Superior De Agronomia - Applicants: Ricardo Manuel De Seixas Boavida Ferreira; Sara Alexandra Valadas Da Silva Monteiro; Artur Ricardo Nascimento Teixeira; Virgilio Borges Loureiro - Inventors: Ricardo Manuel De Seixas Boavida Ferreira; Sara Alexandra Valadas Da Silva Monteiro; Artur Ricardo Nascimento Teixeira; Virgilio Borges Loureiro - Web page: https://patentgrep.com/patent/US8541654B2 ## CPC - C07K 14/415: from plants - A01N 65/00: Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00) - A01N 65/20: Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia - A23K 20/147: Polymeric derivatives, e.g. peptides or proteins - A23L 33/18: Peptides; Protein hydrolysates - A61P 3/02: Nutrients, e.g. vitamins, minerals - C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis} - C12N 15/8282: {for fungal resistance} - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A23K: FODDER - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract This invention is related to a protein from the seeds, cotyledons or plantlets of Lupinus genus, as well as to the way of producing the protein in recombinant form and of expressing the protein in genetically modified plants. The invention relates to methods of its use, or of any modification of the protein that maintains its biological properties, as a supplement in human or animal nutrition and as a fungicide, insecticide, growth promoter, fertilizer or in the preparation of genetically modified organisms. ## Claims ### Claim 1 A method of treating or preventing fungi, Oomycetes, or insects comprising applying to a plant in need thereof an effective amount of a polypeptide, the polypeptide comprising the amino acid sequence of SEQ ID NO: 1 or a sequence having at least 85% sequence identity thereto, wherein the polypeptide does not comprise a sequence of 531 consecutive amino acid residues of β conglutin from Lupinus albus, and wherein the polypeptide exhibits at least one of the group consisting of: anti-fungal activity, anti-Oomyces activity, and insecticide activity. ### Claim 2 (depends on 1) The method of claim 1, wherein the polypeptide comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 3 (depends on 2) The method of claim 2, wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 4 (depends on 1) The method of claim 1, wherein the polypeptide is glycosylated, phosphorylated, alkylated or prenylated. ### Claim 5 (depends on 1) The method of claim 1, wherein the polypeptide is in the form of a formulation, preparation or a crude or partially purified extract from a wild-type or genetically modified plant, or from a culture of transformed cells derived from a plant or microorganism. ### Claim 6 (depends on 1) The method of claim 1, wherein the polypeptide is in the form of a crude extract from a plant of the genus Lupinus. ### Claim 7 (depends on 6) The method of claim 6, wherein the extract is from Lupinus cotyledons. ### Claim 8 A method of promoting plant growth or fertilization comprising applying to a plant in need thereof an effective amount of a polypeptide, the polypeptide comprising the amino acid sequence of SEQ ID NO: 1 or a sequence having at least 85% sequence identity thereto, wherein the polypeptide does not comprise a sequence of 531 consecutive amino acid residues of β conglutin from Lupinus albus, and wherein the polypeptide exhibits at least one of the group consisting of: anti-fungal activity, anti-Oomyces activity, and insecticide activity. ### Claim 9 (depends on 8) The method of claim 8, wherein the polypeptide comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 10 (depends on 9) The method of claim 9, wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 11 (depends on 8) The method of claim 8, wherein the polypeptide is glycosylated, phosphorylated, alkylated or prenylated. ### Claim 12 (depends on 8) The method of claim 8, wherein the polypeptide is in the form of a formulation, preparation or a crude or partially purified extract from a wild-type or genetically modified plant, or from a culture of transformed cells derived from a plant or microorganism. ### Claim 13 (depends on 8) The method of claim 8, wherein the polypeptide is in the form of a crude extract from a plant of the genus Lupinus. ### Claim 14 (depends on 13) The method of claim 13, wherein the extract is from Lupinus cotyledons.