# US 8,541,563 B2 · Plant wall degradative compounds and systems

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- Number: US 8,541,563 B2
- Document ID: US8541563B2
- Status: Granted
- Kind: B2
- Application number: 13/465,714
- Filed: 2012-05-07
- Published: 2012-12-27
- Granted: 2013-09-24
- Earliest claimed: 2004-05-04
- Assignees: University of Maryland
- Applicants: Larry Edmund Taylor; Ronald M. Weiner; Steven Wayne Hutcheson; Nathan A. Ekborg; Michael Howard
- Inventors: Larry Edmund Taylor; Ronald M. Weiner; Steven Wayne Hutcheson; Nathan A. Ekborg; Michael Howard
- Web page: https://patentgrep.com/patent/US8541563B2

## CPC

- C12N 9/2437: {Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)}
- A01N 37/46: N-acyl derivatives
- A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46)
- C12N 9/2402: {hydrolysing O- and S- glycosyl compounds (3.2.1)}
- C12N 9/248: {Xylanases}
- C12N 9/2488: {Mannanases}
- C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase}
- C12Y 302/01004: Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12Y: ENZYMES

## Abstract

The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose. These systems have a number of applications.

## Claims

### Claim 1

An isolated Saccharophagus degradans strain 2-40 polynucleotide encoding an amino acid sequence with arabinofuranosidase activity and comprising a GH43 domain.

### Claim 2

An isolated Saccharophagus degradans strain 2-40 polypeptide with arabinofuranosidase activity and comprising a GH43 domain.

### Claim 3

A vector comprising an isolated Saccharophagus degradans strain 2-40 polynucleotide encoding an amino acid sequence with arabinofuranosidase activity and comprising a GH43 domain.

### Claim 4

A host cell comprising an isolated Saccharophagus degradans strain 2-40 polynucleotide encoding an amino acid sequence with arabinofuranosidase activity and comprising a GH43 domain.

### Claim 5 (depends on 4)

The host cell of claim 4, wherein the cell is an Escherichia coli cell.