# US 8,541,239 B2 · Assay method for detecting primary amines

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- Number: US 8,541,239 B2
- Document ID: US8541239B2
- Status: Granted
- Kind: B2
- Application number: 13/125,286
- Filed: 2009-10-15
- Published: 2011-12-22
- Granted: 2013-09-24
- Earliest claimed: 2008-10-22
- Assignees: Oxtox Limited
- Applicants: Dimitrios Konstantinos Kampouris; Patrick Robinson Huddleston; Craig Edward Banks
- Inventors: Dimitrios Konstantinos Kampouris; Patrick Robinson Huddleston; Craig Edward Banks
- Web page: https://patentgrep.com/patent/US8541239B2

## CPC

- G01N 33/946: {CNS-stimulants, e.g. cocaine, amphetamines}
- G01N 33/523: {the element being adapted for a specific analyte}
- Y10T 436/14: Heterocyclic carbon compound [i.e. , O, S, N, Se, Te, as only ring hetero atom]
- Y10T 436/145555: Hetero-N
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

The invention provides an assay method for the detection of a primary amine analyze in an aqueous body fluid, which method comprises contacting a sample of said body fluid at a pH below 9.5 with a thiol and an unsaturated cis-dialdehyde and testing for the presence of a pyrrole reaction product.

## Claims

### Claim 1

An assay method for the detection of a primary amine analyte in an aqueous body fluid, which method comprises contacting a sample of said body fluid at a pH below 9.5 with a thiol and an unsaturated cis-dialdehyde and testing for the presence of a pyrrole reaction product, wherein the presence of a pyrrole reaction product is tested for electrochemically.

### Claim 2 (depends on 1)

The assay method as claimed in claim 1 wherein said cis-dialdehyde is an o-phthalaldehyde.

### Claim 3 (depends on 1, 2)

The assay method as claimed in claim 1 or claim 2 wherein said body fluid is urine or saliva.

### Claim 4 (depends on 1, 2)

The assay method as claimed in claim 1 or claim 2 wherein said pH below 9.5 is a pH in the range of 6 to 8.

### Claim 5 (depends on 1, 2)

The assay method as claimed in claim 1 or claim 2 wherein said body fluid is also subjected to a further assay capable of detecting both primary and secondary amines.

### Claim 6 (depends on 1, 2)

The assay method as claimed in claim 1 or claim 2 wherein said primary amine analyte is a primary amphetamine.

### Claim 7

An assay cartridge comprising a casing having an aperture for placement of a body fluid sample and containing a water-absorbent pad, a thiol, and an unsaturated cis-dialdehyde, and at least two electrodes capable of being in electrical contact through said pad when said pad is moist.

### Claim 8 (depends on 7)

The assay cartridge as claimed in claim 7 wherein said thiol and unsaturated cis-dialdehyde are impregated into said pad and said cartridge has a radiation translucent window or aperture allowing radiation from said pad to exit said cartridge.

### Claim 9 (depends on 3)

The assay method as claimed in claim 3 wherein said pH below 9.5 is a pH in the range of 6 to 8.

### Claim 10 (depends on 3)

The assay method as claimed in claim 3 wherein said body fluid is also subjected to a further assay capable of detecting both primary and secondary amines.

### Claim 11 (depends on 4)

The assay method as claimed in claim 4 wherein said body fluid is also subjected to a further assay capable of detecting both primary and secondary amines.

### Claim 12 (depends on 3)

The assay method as claimed in claim 3 wherein said primary amine analyte is a primary amphetamine.

### Claim 13 (depends on 4)

The assay method as claimed in claim 4 wherein said primary amine analyte is a primary amphetamine.

### Claim 14 (depends on 5)

The assay method as claimed in claim 5 wherein said primary amine analyte is a primary amphetamine.