# US 8,541,233 B2 · Methods for collecting and processing autografts, processed autografts, kits for collecting and transporting autografts, and tools for preparing autografts

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- Number: US 8,541,233 B2
- Document ID: US8541233B2
- Status: Granted
- Kind: B2
- Application number: 12/825,033
- Filed: 2010-06-28
- Published: 2011-06-30
- Granted: 2013-09-24
- Earliest claimed: 2009-06-26
- Assignees: LifeNet Health
- Applicants: Roberto Bracone; Jingsong Chen; Silvia Chen; James A. Clagett; Marilyn Gatin; Daniel Osborne
- Inventors: Roberto Bracone; Jingsong Chen; Silvia Chen; James A. Clagett; Marilyn Gatin; Daniel Osborne
- Web page: https://patentgrep.com/patent/US8541233B2

## CPC

- A01N 1/124: Disinfecting agents, e.g. antimicrobials
- A61F 2/2875: {Skull or cranium (A61F2/2803 takes precedence; joints for temporo-mandibular [TM] joints [TMJ] A61F2/3099)}
- A61F 2/4644: {Preparation of bone graft, bone plugs or bone dowels, e.g. grinding or milling bone material (bone material A61F2/28; disintegrating or milling devices B02C)}
- A61F 2/28: Bones
- A61F 2310/00359: Bone or bony tissue
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C)

## Abstract

The present invention is directed to methods for collecting and processing autografts, processed autografts, kits for collecting and transporting autografts, and tools for preparing autografts. It is also directed to autologous bone grafts, and methods of preparing them.

## Claims

### Claim 1

A method of preparing a clean, disinfected autologous tissue implant for implantation, the method comprising
a) receiving a treated tissue that has been removed from a patient, wherein the treated tissue is a bone flap,
b) processing the treated tissue to clean and disinfect the tissue, wherein the processing comprises demineralization of the treated tissue, and
c) providing the clean, disinfected autologous tissue to a surgical facility for re-implantation into the patient.

### Claim 2 (depends on 1)

The method of claim 1, wherein the bone flap is a cranial bone flap.

### Claim 3 (depends on 1)

The method of claim 1, wherein the received, treated tissue is a tissue that has been treated with an antibiotic solution, a bactericidal solution, a virucidal, a glycerol-containing solution, water, saline or a combination thereof.

### Claim 4 (depends on 1)

The method of claim 1, wherein the processing comprises at least one cleaning step.

### Claim 5 (depends on 1)

The method of claim 1, wherein the processing comprises debridement.

### Claim 6 (depends on 1)

The method of claim 1, wherein the processing further comprises subjecting the tissue to a plasticizer.

### Claim 7 (depends on 1)

The method of claim 1, wherein the processing further comprises creating an instrument insertion site in the tissue.

### Claim 8 (depends on 1)

The method of claim 1, wherein the processing comprises exposing the tissue to at least one bioactive factor.

### Claim 9 (depends on 1)

The method of claim 1, wherein the tissue is irradiated after processing.

### Claim 10 (depends on 1)

The method of claim 1, further comprising storing the processed tissue in a storage solution.

### Claim 11 (depends on 10)

The method claim 10, wherein the tissue is irradiated while in the storage solution.

### Claim 12 (depends on 1)

The method of claim 1, further comprising seeding cells onto the tissue prior to re-implantation.