# US 8,541,232 B2 · Composition comprising a progenitor/precursor cell population

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- Number: US 8,541,232 B2
- Document ID: US8541232B2
- Status: Granted
- Kind: B2
- Application number: 12/224,913
- Filed: 2007-03-08
- Published: 2010-11-18
- Granted: 2013-09-24
- Earliest claimed: 2006-03-08
- Assignees: Kwalata Trading Limited
- Applicants: Yael Porat; Valentin Fulga; Svetlana Porozov; Adina Belleli
- Inventors: Yael Porat; Valentin Fulga; Svetlana Porozov; Adina Belleli
- Web page: https://patentgrep.com/patent/US8541232B2

## CPC

- C12N 5/0692: {Stem cells; Progenitor cells; Precursor cells}
- A61K 35/44: Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
- C12N 5/0607: {Non-embryonic pluripotent stem cells, e.g. MASC (induced pluripotent stem cells C12N5/0696)}
- C12N 5/0634: {Cells from the blood or the immune system}
- A61K 2035/124: {the cells being hematopoietic, bone marrow derived or blood cells}
- C12N 2501/14: Erythropoietin [EPO]
- C12N 2501/165: Vascular endothelial growth factor [VEGF]
- C12N 2501/23: Interleukins [IL]
- C12N 2501/33: Insulin
- C12N 2501/39: Steroid hormones
- C12N 2501/392: Sexual steroids
- C12N 2501/599: with CD designations not provided for elsewhere
- C12N 2501/91: Heparin
- C12N 2506/11: from blood or immune system cells
- C12N 2510/00: Genetically modified cells
- C12N 2533/52: Fibronectin; Laminin
- C12N 2533/54: Collagen; Gelatin
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

A population of cultured cells is provided that comprises at least 10% of a sub-population of cells that: stain as CD31 Bright, demonstrate uptake of Ac-LDL+, and secrete both IL-8 and angiogenin.

## Claims

### Claim 1

A composition of matter, comprising a population of cultured cells that comprises a sub-population of cells that stain as CD31Bright, demonstrate uptake of Ac-LDL+ and secrete interleukin-8 and angiogenin, wherein the sub-population of cells comprises at least 10% of the cells in the population of cultured cells.

### Claim 2 (depends on 1)

The composition of matter according to claim 1, wherein the sub-population secretes at least 50 pg interleukin-8 per 106 cells/ml over a period of at least 24 hours.

### Claim 3 (depends on 1)

The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population comprise a feature selected from the group consisting of: CD34, CD117, CD133, Tie-2, CD34+CD133+, KDR, CD34+KDR+, CD144, von Willebrand Factor, SH2 (CD105), SH3, fibronectin, collagen type I, collagen type III, collagen type IV, ICAM type 1, ICAM type 2, VCAM1, vimentin, BMP-R IA, BMP-RII, CD44, integrin b1, aSM-actin, MUC18, CXCR4 and CXCR8.

### Claim 4 (depends on 1)

The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population secrete a molecule selected from the group consisting of: vascular endothelial growth factor, matrix metalloproteinase-2, and matrix metalloproteinase-9.

### Claim 5 (depends on 1)

The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population include a feature selected from the group consisting of: a tube-like structure, a tendency to form a colony, a tendency to form a cluster, and a tendency to migrate toward a chemoattractant.

### Claim 6 (depends on 1)

The composition of matter according to claim 1, wherein the sub-population comprises at least 25% of the cells in the population.

### Claim 7 (depends on 1)

The composition of matter according to claim 1, wherein the sub-population comprises at least 50% of the cells in the population.

### Claim 8 (depends on 1)

The composition of matter according to claim 1, wherein the sub-population secretes at least 150 pg IL-8 per 106 cells/ml over a period of at least 24 hours.

### Claim 9 (depends on 1)

The composition of matter according to claim 1, wherein the sub-population secretes at least 1000 pg IL-8 per 106 cells/ml over a period of at least 24 hours.

### Claim 10 (depends on 1)

The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population include a morphological feature selected from the group consisting of: a cell size larger than 20 μm, an elongated cell, a spindle-shaped cell, an irregularly-shaped cell, a granulated cell, a cell including an enlarged dark nucleus, a multinuclear cell, a cell including flagella-like structures, a cell including pseudopodia, and a cell having a polygonal shape.

### Claim 11 (depends on 1)

The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population have a tendency to migrate toward a chemoattractant selected from the group consisting of: bFGF, VEGF, SCF, G-CSF, GM-CSF, and SDF-1.

### Claim 12 (depends on 1)

The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population have a tendency to migrate toward IL-8.