# US 8,541,228 B2 · Cell observation apparatus, cell observation method, and program > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,541,228 B2 - Document ID: US8541228B2 - Status: Granted - Kind: B2 - Application number: 12/451,384 - Filed: 2008-06-20 - Published: 2010-05-27 - Granted: 2013-09-24 - Earliest claimed: 2007-06-22 - Assignees: Nikon Corporation - Applicants: Yasujiro Kiyota - Inventors: Yasujiro Kiyota - Web page: https://patentgrep.com/patent/US8541228B2 ## CPC - G02B 21/34: Microscope slides, e.g. mounting specimens on microscope slides - C12M 41/14: {Incubators; Climatic chambers (per se B01L1/00)} - C12M 41/36: {of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404)} - C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)} - G01N 35/0092: {Scheduling} - G02B 21/365: {Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence)} - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present invention relates to a cell observation apparatus that facilitates the setting of schedules. An observation schedule that has already been registered is presented to a user by a time schedule display unit of a schedule setting screen, and a new observation schedule is acquired in response to an input of set values of photographic conditions by the user. Then determination is made whether the photographing time of this observation schedule and the photographing time of the already registered observation schedule overlap, and when the photographing times are determined to overlap, the photographic conditions including the photographing time of one or both of the observation schedules are changed and the observation schedule is registered. The invention can be applied, for example, to incubators for culturing cells. ## Claims ### Claim 1 A cell observation apparatus for observing a culturing process of cells according to an observation schedule designated by a user, the apparatus comprising: a cell culture incubator with an operation control unit; a photographic unit that photographs the cells according to the observation schedule; at least one input unit within the control unit that acquires a new observation schedule in response to an input of photographic conditions by the user; at least one CPU (Central Processing Unit) within the control unit that determines whether a photographing time of the photographic unit included in the photographic conditions of the new observation schedule that has been acquired by the at least one input unit overlaps a photographing time of the photographic unit included in the photographic conditions of an already registered observation schedule and changes the photographic conditions of either one or both of the observation schedules when the overlapping of the photographing times of the observation schedules has been determined by the at least one CPU; a data storage unit within the control unit that registers the new observation schedule, or registers the new observation schedule and then re-registers the already registered observation schedule on the basis of the photographic conditions that are changed by the at least one CPU; and the cell culture incubator further comprises a stocker unit and a conveying unit. ### Claim 2 (depends on 1) The cell observation apparatus according to claim 1, wherein the photographic conditions of the observation schedule include at least a photographing frequency of the photographic unit in time-lapse photography, a photographing time, and a photographing interval; the at least one CPU changes the photographic conditions of the observation schedule by shifting one or both of the photographing times, which have been determined to overlap by the at least one CPU, back or forth within an allowed time range that has been set for the respective observation schedule; and the storage unit registers the observation schedule in a case where the overlapping of photographing times of the observation schedules has been eliminated by changing the photographic conditions by the CPU. ### Claim 3 (depends on 2) The cell observation apparatus according to claim 2, wherein the allowed time, which is a time in which shifting of the photographing time by the at least one CPU is allowed, is determined by automatic computation of a predetermined ratio corresponding to the photographing interval or by manual input thereof. ### Claim 4 (depends on 2) The cell observation apparatus according to claim 2, further comprising: an operation panel, wherein the operation panel displays an overlapping portion of the observation schedules after the change made by the at least one CPU, and in a case where the overlapping of photographing times of the observation schedules has not been eliminated despite the change in the photographic conditions of the observation schedule made by the least one CPU, the at least one input unit acquires a new observation schedule again in response to a change in the photographic conditions made by the user. ### Claim 5 (depends on 1) The cell observation apparatus according to claim 1, further comprising: an operation panel, wherein the operation panel displays an overlapping state of the photographing times to the user when the photographing times have been determined to overlap by the at least one CPU, and the at least one CPU acquires a new observation schedule again in response to a change in the photographic conditions made by the user. ### Claim 6 (depends on 1) The cell observation apparatus according to claim 1, wherein; a priority between a plurality of users is registered in the storage unit, the at least one CPU determines the priority between a plurality of users, and the at least one CPU changes the photographic conditions of an observation schedule of the user with a lower priority that has been registered in the storage unit by the at least one CPU when the observation schedules, for which the photographing times have been determined to overlap by the at least one CPU, have different users. ### Claim 7 (depends on 1) The cell observation apparatus according to claim 1, wherein the at least one CPU sets a time required for the photographic unit to photograph, so that photographing by the photographic unit is completed within the photographing time of a new observation schedule that has been acquired by the at least one input unit; the at least one input unit acquires, in response to input by a user, a photographic magnification at which the cells are photographed and a Z stack number in a direction of an optical axis in which the cells are photographed; the at least one CPU compounds a basic photographing time that is a time necessary to photograph the Z stack number that has been acquired by the at least one input unit at the photographic magnification acquired by the at least one input unit; the at least one input unit acquires, in response to input by a user, the number of photographic points per plane perpendicular to an optical axis; the at least one CPU computes a required photographing time that is a time required to photograph the number of photographic points that have been acquired by the at least one input unit, within the basic photographing time that has been calculated by the at least one CPU; and the at least one CPU determines whether the required photographing time calculated by the at least one CPU is within the photographing time of a new observation schedule that has been acquired by the storage unit, and wherein in a case where the required photographing time is determined by the at least one CPU not to be within the photographing time of a new observation schedule that has been acquired by the storage unit, either the acquisition of the photographic magnification and the Z stack number by the at least one input unit or the acquisition of the number of photographic points by the at least one input unit is conducted again. ### Claim 8 A cell observation method for observing a culturing process of cells according to an observation schedule by which time lapse observations with a predetermined photographing time are performed at every predetermined photographing interval, the method comprising the steps of: presenting to a user an observation schedule that has already been registered; acquiring a photographing interval and a photographing time of a new observation schedule in response to an input of photographic conditions by the user; determining whether photographing times of the new observation schedule and the already registered observation schedule overlap on the basis of the photographing interval and the photographing time; and changing, in a case where the photographing times have been determined to overlap, the photographic conditions of the observation schedule by shifting the photographing time of one or both of the observation schedules back or forth within an allowed time range that has been set in advance, and registering the observation schedule.