# US 8,541,222 B2 · Modified microorganisms with inactivated lactate dehydrogenase gene

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- Number: US 8,541,222 B2
- Document ID: US8541222B2
- Status: Granted
- Kind: B2
- Application number: 11/915,930
- Filed: 2006-06-07
- Published: 2009-08-06
- Granted: 2013-09-24
- Earliest claimed: 2005-06-07
- Assignees: TMO Renewables Limited
- Applicants: Anthony Atkinson; Roger Cripps; Ann Thompson; Kirstin Eley; Mark Taylor; David Leak; Brian Rudd; Simon Baker
- Inventors: Anthony Atkinson; Roger Cripps; Ann Thompson; Kirstin Eley; Mark Taylor; David Leak; Brian Rudd; Simon Baker
- Web page: https://patentgrep.com/patent/US8541222B2

## CPC

- C12P 7/065: {with microorganisms other than yeasts}
- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

Modified microorganisms are prepared by inactivation of the endogenous lactate dehydrogenase gene. The microorganisms are deposited under NCIMB Accession Nos. 41277, 41278, 41279, 41280 or 41281.

## Claims

### Claim 1

A microorganism deposited under NCIMB number 41277, 41278, 41279, 41280, or 41281, wherein the microorganism has been modified to delete the endogenous lactate dehydrogenase gene.

### Claim 2 (depends on 1)

The microorganism of claim 1, wherein the microorganism is deposited under NCIMB number 41277.

### Claim 3 (depends on 1)

The microorganism of claim 1, wherein the microorganism is deposited under NCIMB number 41278.

### Claim 4 (depends on 1)

The microorganism of claim 1, wherein the microorganism is deposited under NCIMB number 41279.

### Claim 5 (depends on 1)

The microorganism of claim 1, wherein the microorganism is deposited under NCIMB number 41280.

### Claim 6 (depends on 1)

The microorganism of claim 1, wherein the microorganism is deposited under NCIMB number 41281.