# US 8,541,217 B2 · Cytoblock preparation system and methods of use

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 8,541,217 B2
- Document ID: US8541217B2
- Status: Granted
- Kind: B2
- Application number: 13/439,559
- Filed: 2012-04-04
- Published: 2012-08-02
- Granted: 2013-09-24
- Earliest claimed: 2006-03-20
- Assignees: Rongshan Li
- Applicants: Rongshan Li; Wu Li
- Inventors: Rongshan Li; Wu Li
- Web page: https://patentgrep.com/patent/US8541217B2

## CPC

- G01N 1/36: Embedding or analogous mounting of samples
- C12M 23/42: {Integrated assemblies, e.g. cassettes or cartridges}
- C12M 33/04: {by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02)}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

An apparatus and method that may be used for collecting target cells or tissue and preparing a cell block are disclosed.

## Claims

### Claim 1

A method of preparing a cytoblock comprising:
a. preparing a cell pellet in a centrifuge tube having a flattened floor;
b. combining the prepared cell pellet with a matrix material in the centrifuge to form a cell mixture;
c. transferring the cell mixture from the centrifuge tube into a tissue cassette having at least one integrally formed chamber that is compatible in size and shape with the bottom region of the centrifuge tube having the flattened floor and processing the mixture; and
d. embedding the processed cell mixture in an embedding block.

### Claim 2 (depends on 1)

The method of claim 1, wherein the preparing further comprises:
a. depositing cell material in the centrifuge tube;
b. adding a predetermined amount of fixative to the centrifuge tube;
c. placing the centrifuge tube in a centrifuge device to create the cell pellet and supernatant; and
d. removing the supernatant from the centrifuge tube.

### Claim 3 (depends on 1)

The method of claim 1, wherein the centrifuge tube further comprises a tapered region and a non-tapered, reduced diameter bottom region.

### Claim 4 (depends on 1)

The method of claim 1, wherein the tissue cassette includes a removable insert having at least one integrally formed chamber.

### Claim 5 (depends on 1)

The method of claim 1, wherein the preparing further comprises depositing cell material in a centrifuge tube containing a predetermined amount of fixative, placing the centrifuge tube in a centrifuge device to create the cell pellet and supernatant; and removing the supernatant from said centrifuge tube.

### Claim 6 (depends on 5)

The method of claim 5, wherein the cell material is obtained by fine needle aspiration.

### Claim 7 (depends on 5)

The method of claim 5, wherein the cell material is obtained by endoscopy.

### Claim 8 (depends on 5)

The method of claim 5, wherein the cell material is obtained by lavage.

### Claim 9 (depends on 5)

The method of claim 5, wherein the cell material is obtained by a catheter.

### Claim 10 (depends on 1)

The method of claim 1 wherein the combining further comprises:
a. providing a matrix container containing the matrix material, the matrix container having the form of a syringe;
b. heating the matrix container to liquefy the matrix material;
c. depositing a predetermined amount of liquefied matrix material into the centrifuge tube;
d. mixing the cell pellet and the matrix material to create a suspension; and
e. cooling the centrifuge tube to solidify the matrix material and create a cell mixture.

### Claim 11 (depends on 1)

The method of claim 1, wherein the embedding further comprises:
a. transferring at least a portion of the cell mixture from the tissue cassette to an embedding block made of wax and formed with at least one hole therein to receive the at least a portion of the cell mixture;
b. placing an embedding tray over the embedding block and inverting the embedding tray and embedding block;
c. warming the embedding tray and embedding block to at least partially melt the embedding block and embed the cell mixture in the embedding block; and
d. cooling the embedding tray and embedding block to solidify the embedding block.

### Claim 12 (depends on 1)

The method of claim 1, wherein the embedding further comprises:
a. transferring at least a portion of the cell mixture from the tissue cassette to an embedding tray, the embedding tray being provided with a partitioned insert formed with at least one partition therein to receive the at least a portion of the cell mixture;
b. depositing a predetermined amount of embedding material into the embedding tray to embed the cell mixture into an embedding block, said embedding material being heated to liquefy the embedding material; and
c. cooling the embedding tray to solidify the embedding block.

### Claim 13 (depends on 1)

The method of claim 1, wherein the embedding further comprises:
a. transferring at least a portion of the cell mixture from the tissue cassette to an embedding tray, the embedding tray provided with liquefied embedding material therein and a portioned insert formed with at least one partition therein to receive the at least a portion of the cell mixture; and
b. cooling the embedding tray to create a solidified embedded block.

### Claim 14 (depends on 1)

The method of claim 1, wherein the tissue cassette includes a fixed insert having at least one integrally formed chamber.