# US 8,541,216 B2 · Insecticidal fermentation broth from actinomycetes containing enhanced ratio of active to inactive dunaimycins

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- Number: US 8,541,216 B2
- Document ID: US8541216B2
- Status: Granted
- Kind: B2
- Application number: 12/939,991
- Filed: 2010-11-04
- Published: 2011-05-12
- Granted: 2013-09-24
- Earliest claimed: 2009-11-06
- Assignees: Bayer CropScience AG
- Applicants: Hong Zhu; Jorge Jimenez; Colleen Taylor; Magalie Guilhabert-Goya; Jonathan Margolis
- Inventors: Hong Zhu; Jorge Jimenez; Colleen Taylor; Magalie Guilhabert-Goya; Jonathan Margolis
- Web page: https://patentgrep.com/patent/US8541216B2

## CPC

- C12P 17/181: {Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin}
- A01N 43/90: having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
- A01N 63/28: Streptomyces
- C12N 1/20: Bacteria; Culture media therefor
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides an insecticidal fermentation broth of an actinomycete, where the fermentation broth contains an optimized ratio of active dunaimycins to inactive dunaimycins.

## Claims

### Claim 1

A composition comprising an insecticidal fermentation broth or solid from an actinomycete wherein the fermentation broth or solid comprises an optimized ratio of active dunaimycins to inactive dunaimycins whereby the optimized ratio provides insecticidal activity at less than 1000 ppm.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the optimized ratio of active dunaimycins to inactive dunaimycins is at least about 1:1.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the concentration of active dunaimycins and inactive dunaimycins is at least about 1 g/L of fermentation broth.

### Claim 4 (depends on 1)

The composition of claim 1, wherein the concentration of active dunaimycins and inactive dunaimycins is at least about 3 g/L of fermentation broth.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the concentration of active dunaimycins and inactive dunaimycins is at least about 7 g/L of fermentation broth.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the actinomycete is a Streptomyces sp, strain that produces both active dunaimycins and inactive dunaimycins.

### Claim 7 (depends on 6)

The composition of claim 6, wherein the Streptomyces sp, strain is Streptomyces galbus.

### Claim 8 (depends on 6)

The composition of claim 6, wherein the Streptomyces sp, strain is Streptomyces sp. M1064.

### Claim 9 (depends on 1)

The composition of claim 1, further comprising a carrier.

### Claim 10 (depends on 1)

The composition of claim 1, wherein the active dunaimycins have a vinylogous enol ether or hemiacetal or acetal moiety at position C18-C19 and the inactive dunaimycins have a ketone at position C19.

### Claim 11 (depends on 10)

The composition of claim 10, wherein the active dunaimycin is one or more of C2, C2S, D2, D2S and D3S and the inactive dunaimycin is one or more of A1 and C1.