# US 8,541,215 B2 · Microorganisms, selenium acid compound-reducing agent, method for reducing and method for removing selenium acid compound, and process for producing metallic selenium

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- Number: US 8,541,215 B2
- Document ID: US8541215B2
- Status: Granted
- Kind: B2
- Application number: 12/737,166
- Filed: 2009-06-17
- Published: 2011-08-25
- Granted: 2013-09-24
- Earliest claimed: 2008-06-18
- Assignees: Electric Power Development Co., Ltd.
- Applicants: Mitsufumi Matsumoto; Yasuhiko Nishimura
- Inventors: Mitsufumi Matsumoto; Yasuhiko Nishimura
- Web page: https://patentgrep.com/patent/US8541215B2

## CPC

- C12P 3/00: Preparation of elements or inorganic compounds except carbon dioxide {(recovery of carbon dioxides as by-products C12F3/02)}
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/205: Bacterial isolates
- C12P 39/00: Processes involving microorganisms of different genera in the same process, simultaneously
- C22B 3/18: with the aid of microorganisms or enzymes, e.g. bacteria or algae
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12R 2001/22: Klebsiella
- Y02P 10/20: Recycling
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C22B: PRODUCTION AND REFINING OF METALS (electrolytic C25); PRETREATMENT OF RAW MATERIALS
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

Disclosed are novel microorganisms having an excellent ability to reduce selenium acid compounds, a selenium acid compound-reducing agent comprising such microorganism(s) as an active ingredient, a method for reducing and a method for removing a selenium acid compound with use of such microorganism(s), and a process for producing metallic selenium. The microorganisms of the present invention are Aeromonas sp. strain JPCC SEP JP-1, Klebsiella sp. strain JPCC SEP JP-2, and Sulfurospirillum sp. strain JPCCY SEP-3. Also disclosed are a method for reducing a selenium acid compound comprising the step of co-culturing these microorganisms under the presence of a selenium acid compound, and a process for producing metallic selenium comprising the steps of: co-culturing these microorganisms under the presence of a selenium acid compound; and separating the thus produced culture product.

## Claims

### Claim 1

A method for reducing a selenium acid compound, comprising the step of co-culturing Aeromonas JPCC SEP JP-1 (accession number NITE BP-345), Klebsiella JPCC SEP JP-2 (accession number NITE BP-346), and Sulfurospirillum JPCCY SEP-3 (accession number NITE BP-582), under the presence of a selenium acid compound.

### Claim 2

A method for reducing a selenium acid compound, comprising the step of culturing Sulfurospirillum JPCCY SEP-3 (accession number NITE BP-582) under the presence of a selenium acid compound.

### Claim 3 (depends on 1)

The method for reducing a selenium acid compound according to claim 1, wherein the step of co-culturing is performed under an anaerobic condition.

### Claim 4 (depends on 3)

The method for reducing a selenium acid compound according to claim 3, wherein the step of co-culturing is performed at 28° C.

### Claim 5 (depends on 2)

The method for reducing a selenium acid compound according to claim 2, wherein the step of co-culturing is performed under an anaerobic condition.

### Claim 6 (depends on 5)

The method for reducing a selenium acid compound according to claim 5, wherein the step of co-culturing is performed at 28° C.