Mutant penicillin G acylases
AssigneeDSM Sinochem Pharmaceuticals Netherlands B.V.
InventorsJan Metske Van Der Laan, Harold Monro Moody, Richard Kerkman, Thomas Van Der Does
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Abstract
The present invention relates to a mutant prokaryotic penicillin G acylase derived from a wild-type penicillin G acylase characterized in that the mutant is having an amino acid substitution at one or more amino acid positions selected from the group consisting of amino acid positions A3, A77, A90, A144 A192, B24, B109, B148, B313, B460 and B488 according to the amino acid numbering of the Escherichia coli penicillin G acylase having the amino acid sequence depicted in SEQ ID No: 1.
Claims13
A mutant prokaryotic penicillin G acylase derived from a wild-type penicillin G acylase characterized in that the mutant comprises an amino acid substitution at least at position B460 and of at least one amino acid position selected from the group consisting of amino acid positions A3, A77, A90, A144, A192, B24, B109, B148, B313, B460 and B488 according to the amino acid numbering of the Escherichia coli penicillin G acylase with the amino acid sequence depicted in SEQ ID NO:
- 1,
- wherein the Escherichia coli penicillin G acylase consists of
- (a) a signal peptide (26 amino acids—amino acid position 1-26);
- (b) an α-subunit (209 amino acids—amino acid position 27-235);
- (c) a connecting peptide (54 amino acids—amino acid position 236-289);
- and
- (d) a β-subunit (557 amino acid position amino acid position 290-846);
- and wherein in the α-subunit the amino acids are numbered A1-A209, in the β-subunit from B1 to B557 and wherein the wild type penicillin G acylase is having an amino acid sequence which is 80-100% homologous to the amino acid sequence depicted in SEQ ID NO:1.
Dependencies are inferred from the claim text.