# US 8,541,181 B2 · Myosin-IIA S1943 phosphorylation as a marker of tumor invasion

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- Number: US 8,541,181 B2
- Document ID: US8541181B2
- Status: Granted
- Kind: B2
- Application number: 13/419,805
- Filed: 2012-03-14
- Published: 2012-09-20
- Granted: 2013-09-24
- Earliest claimed: 2011-03-16
- Assignees: Albert Einstein College of Medicine of Yeshiva University
- Applicants: Anne Reba Bresnick
- Inventors: Anne Reba Bresnick
- Web page: https://patentgrep.com/patent/US8541181B2

## CPC

- G01N 33/5758: {involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites}
- G01N 33/573: for enzymes or isoenzymes
- G01N 33/57515: of the breast
- G01N 2333/4712: Muscle proteins, e.g. myosin, actin, protein
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Methods and kits are provided for determining if cells of a tumor in an organ or a tissue in a subject are likely to invade another organ or tissue in the subject and for determining if a cancer in a subject is likely to metastasize.

## Claims

### Claim 1

A method for determining if cells of a tumor in an organ or in a tissue in a subject, are likely to invade another organ or tissue in the subject comprising determining if a sample of the tumor, or a sample obtained from the tumor, comprises human myosin-IIA heavy chain phosphorylated at residue S1943, comprising contacting the sample of the tumor, or the sample obtained from the tumor, with a detectable agent which comprises an antibody or an antigen-binding fragment of an antibody, which selectively binds to phosphorylated S1943 residue of human myosin-IIA heavy chain and detecting any bound detectable agent,
wherein cells of the tumor are likely to invade another organ or tissue if the sample comprises the phosphorylated S1943 residue of human myosin-IIA heavy chain, and wherein absence of a phosphorylated S1943 residue of human myosin-IIA heavy chain does not indicate that cells of the tumor are likely to invade another organ or tissue.

### Claim 2 (depends on 1)

The method of claim 1, wherein the human myosin-IIA heavy chain comprises SEQ ID NO:1.

### Claim 3 (depends on 1)

The method of claim 1, wherein the detectable agent is an antibody.

### Claim 4 (depends on 3)

The method of claim 3, wherein the antibody is labeled with a detectable label.

### Claim 5 (depends on 4)

The method of claim 4, wherein the detectable label is fluorescent, is radioactive, or is radio-opaque.

### Claim 6 (depends on 1)

The method of claim 1, wherein the phosphorylated S1943 residue of the human myosin-IIA heavy chain is detected on the surface of a cell of the sample.

### Claim 7 (depends on 1)

The method of claim 1, wherein the sample obtained from the tumor is not a lysate.

### Claim 8 (depends on 1)

The method of claim 1, wherein the tumor is a breast cancer tumor.

### Claim 9

A method for determining if a cancer is likely to metastasize comprising determining if a sample of the cancer, or a sample obtained from the cancer, comprises human myosin-IIA heavy chain phosphorylated at residue S1943 comprising contacting the sample of the cancer, or the sample obtained from the cancer, with a detectable agent which comprises an antibody or an antigen-binding fragment of an antibody which selectively binds to phosphorylated S1943 residue of myosin-IIA heavy chain and detecting any bound detectable agent,
wherein the cancer is likely to metastasize if the sample comprises the phosphorylated S1943 residue of human myosin-IIA heavy chain.