# US 8,541,168 B1 · Multi-array, multi-specific electrochemiluminescence testing

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- Number: US 8,541,168 B1
- Document ID: US8541168B1
- Status: Granted
- Kind: B1
- Application number: 09/677,799
- Filed: 2000-10-02
- Granted: 2013-09-24
- Earliest claimed: 1995-03-10
- Applicants: Jacob Wohlstadter; James Wilbur; George Sigal; Mark Martin; Liang-Hong Guo; Alan Fischer; Jon Leland
- Inventors: Jacob Wohlstadter; James Wilbur; George Sigal; Mark Martin; Liang-Hong Guo; Alan Fischer; Jon Leland
- Web page: https://patentgrep.com/patent/US8541168B1

## CPC

- G01N 21/66: electrically excited, e.g. electroluminescence
- G01N 21/76: Chemiluminescence; Bioluminescence
- G01N 33/5438: {Electrodes}
- G01N 33/582: {with fluorescent label}
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- Y10S 435/808: Optical sensing apparatus
- Y10S 435/968: High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
- Y10S 435/973: Simultaneous determination of more than one analyte
- Y10S 436/805: Optical property
- Y10S 436/806: Electrical property or magnetic property
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Materials and methods are provided for producing patterned multi-array, multi-specific surfaces which are electronically excited for use in electrochemiluminescence based tests. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use in flat panel displays and multiply specific testing procedures.

## Claims

### Claim 1

A method for detecting a nucleic acid by hybridization reaction comprising:
(a) forming a composition comprising a first nucleic acid and a second nucleic acid, wherein said second nucleic acid is immobilized on an electrode and covalently attached to a label, and
(b) measuring the electron transfer between the label and the electrode so as to determine if said first nucleic acid has hybridized to said second nucleic acid, wherein electron transfer rate is increased by hybridization;
wherein said electrode comprises a mixed monolayer and at least one constituent of said mixed monolayer facilitates the transfer of electrical potential.

### Claim 2 (depends on 1)

The method of claim 1, wherein the electron transfer between the label and the electrode is measured via an electrochemiluminescence measurement.

### Claim 3 (depends on 1)

The method of claim 1, wherein the second nucleic acid is 5′ thiolated DNA immobilized on said electrode.

### Claim 4 (depends on 3)

The method of claim 3, wherein said electrode is a gold electrode.

### Claim 5 (depends on 1)

The method of claim 1, wherein said second nucleic acid is coupled to said electrode via a linker with a length less than 10 atoms.

### Claim 6 (depends on 1)

The method of claim 1, wherein said second nucleic acid is coupled to said electrode via a conductive linker.

### Claim 7 (depends on 6)

The method of claim 6, wherein said conductive linker comprises polyacetylene.