# US 8,541,032 B2 · Tissue graft composition > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,541,032 B2 - Document ID: US8541032B2 - Status: Granted - Kind: B2 - Application number: 13/649,522 - Filed: 2012-10-11 - Published: 2013-02-07 - Granted: 2013-09-24 - Earliest claimed: 2009-07-22 - Assignees: ACell, Inc. - Applicants: Miles Grody - Inventors: Rodney W. Bosley, Jr.; Clay Fette - Web page: https://patentgrep.com/patent/US8541032B2 ## CPC - A61L 27/3633: {Extracellular matrix [ECM]} - A61F 2/02: Prostheses implantable into the body - A61L 27/3604: {characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel} - A61L 27/50: Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials} - A61F 2210/0004: bioabsorbable - A61F 2210/0076: multilayered, e.g. laminated structures - A61L 2430/34: for soft tissue reconstruction - Y10T 156/10: Methods of surface bonding and/or assembly therefor - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract Disclosed are tissue graft compositions made of materials having different densities, methods of making, and methods of treatment for restoring tissues in a patient. ## Claims ### Claim 1 A method for restoring a tissue at an anatomic site of a patient, comprising: providing a composition comprising an epithelial basement membrane and, an extracellular matrix material comprising a devitalized portion of a site-specific extracellular matrix material derived from an anatomic site of a mammalian source other than said patient wherein said site-specific extracellular matrix material corresponds to said anatomic site in need of restoration of said patient, and, one of said epithelial basement membrane and said extracellular matrix material has a plurality of raised elements and the other of said epithelial basement membrane and said extracellular matrix material has a plurality of depressions, wherein said raised elements are introduced into said depressions to join the epithelial basement membrane to said extracellular matrix material; and implanting said composition at the anatomic site of the patient. ### Claim 2 (depends on 1) The method of claim 1, wherein said epithelial basement membrane is located on a surface of said composition. ### Claim 3 (depends on 1) The method of claim 1 wherein said composition further comprises more than one layer of said site-specific extracellular matrix material. ### Claim 4 (depends on 1) The method of claim 1, wherein the composition is a tissue graft composition comprising said epithelial basement membrane on one surface of the tissue graft composition and said extracellular matrix material on another surface of said tissue graft composition. ### Claim 5 (depends on 1) The method of claim 1, wherein said extracellular matrix material comprises aponeurosis. ### Claim 6 (depends on 1) The method of claim 1, wherein said site-specific extracellular matrix material comprises an extracellular matrix of skin. ### Claim 7 (depends on 1) The method of claim 1, wherein said site-specific extracellular matrix material comprises an extracellular matrix material of intestine. ### Claim 8 (depends on 1) The method of claim 1, wherein said site-specific extracellular matrix material comprises an extracellular matrix material of urinary bladder. ### Claim 9 (depends on 1) The method of claim 1, wherein said site-specific extracellular matrix material comprises an extracellular matrix material of a tissue selected from the group consisting of smooth muscle and striated muscle. ### Claim 10 (depends on 1) The method of claim 1 further comprising an extracellular matrix material of tendon. ### Claim 11 (depends on 1) The method of claim 1 further comprising an extracellular matrix material of cartilage. ### Claim 12 (depends on 1) The method of claim 1 further comprising an extracellular matrix material of smooth muscle. ### Claim 13 (depends on 1) The method of claim 1 further comprising an extracellular matrix material of striated muscle. ### Claim 14 (depends on 1) The method of claim 1 further comprising an extracellular matrix material of aponeurosis. ### Claim 15 (depends on 1) The method of claim 1 wherein said depressions comprise valleys. ### Claim 16 (depends on 1) The method of claim 1 wherein said depressions comprise cups. ### Claim 17 (depends on 1) The method of claim 1 wherein said depressions comprise dimples. ### Claim 18 (depends on 1) The method of claim 1 wherein said depressions comprise cylinders.