# US 8,541,017 B2 · Use of alginate matrices to control cell growth

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- Number: US 8,541,017 B2
- Document ID: US8541017B2
- Status: Granted
- Kind: B2
- Application number: 12/854,999
- Filed: 2010-08-12
- Published: 2010-12-02
- Granted: 2013-09-24
- Earliest claimed: 2003-12-23
- Assignees: FMC Biopolymer AS
- Applicants: Jan Egil Melvik; Michael Dornish
- Inventors: Jan Egil Melvik; Michael Dornish
- Web page: https://patentgrep.com/patent/US8541017B2

## CPC

- A61L 27/3886: {comprising two or more cell types}
- A61L 27/20: Polysaccharides
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Methods of inhibiting proliferation of a plurality of proliferating cells are disclosed. Methods of inhibiting cell overgrowth on compositions that are in an animal's body are disclosed. Methods of inhibiting cell overgrowth on a device that is in an animal's body are disclosed. Devices that have on their exterior surface an alginate matrix that comprises Strontium are disclosed. Compositions comprising an alginate body and alginate sheets that each comprise a single layer of cells coating the exterior surface of the alginate body are disclosed. Methods of preparing an artificial tissue are disclosed. Devices comprising cells encapsulated within an alginate matrix and/or maintained as a monolayer on an alginate body, and methods of making and using the same are disclosed. Methods of coating compositions and devices are disclosed.

## Claims

### Claim 1

A method of preparing an artificial tissue comprising the steps of:
growing a plurality of sheets of cells consisting of a single layer of cells on each sheet and each sheet consists of alginate polymer and at least one of calcium, barium, and zinc;
stacking the sheets of cells by placing the bottom of one sheet on the cells of another sheet of cells;
maintaining the stacked sheets of cells under conditions in which the alginate matrix of each sheet is dissolved whereby each single layer of cells comes in direct contact with at least one other single layer of cells to produce a tissue having a plurality of layers of cells.

### Claim 2 (depends on 1)

The method of claim 1 wherein the cell layers comprise one or more layers of fibroblast cells and/or a plurality of layers of epithelial cells and/or a plurality of layers of keratinocytes.

### Claim 3 (depends on 1)

The method of claim 1, wherein said sheet consists of alginate polymer and calcium.