# US 8,541,015 B1 · Three-dimensional biological scaffold and method of making the same

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- Number: US 8,541,015 B1
- Document ID: US8541015B1
- Status: Granted
- Kind: B1
- Application number: 13/560,932
- Filed: 2012-07-27
- Granted: 2013-09-24
- Earliest claimed: 2008-04-24
- Assignees: HRL Laboratories, LLC
- Applicants: David Kisailus; Alan J. Jacobsen; Chaoyin Zhou
- Inventors: David Kisailus; Alan J. Jacobsen; Chaoyin Zhou
- Web page: https://patentgrep.com/patent/US8541015B1

## CPC

- C12M 25/14: {Scaffolds; Matrices (in general C12N5/0068)}
- A61L 27/30: Inorganic materials
- A61L 27/56: Porous materials, {e.g. foams or sponges}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

A three-dimensional biological scaffold. The scaffold includes at least three sets of polymer waveguides extending along at least three respective directions. The at least three sets of polymer waveguides interpenetrate each other at a plurality of nodes to form a self-supporting structure. In some embodiments, the polymer waveguides may be bio-degradable. In still some embodiments, the three-dimensional biological scaffold may include one or more coating layers for covering surfaces of the polymer waveguides.

## Claims

### Claim 1

A method for forming a three-dimensional biological scaffold having a plurality of ordered interconnected pores, the method comprising:
placing a mask having a plurality of apertures between at least one collimated light source and a volume of photo-monomer, in a first position relative to the volume of photo-monomer;
directing a plurality of collimated light beams from the at least one collimated light source through the plurality of apertures into a portion of the volume of photo-monomer, while the mask remains in the first position relative to the volume of photo-monomer, to polymerize the portion to form a plurality of bio-degradable polymer waveguides; and
removing photo-monomer of the volume of photo-monomer not polymerized to leave behind the three-dimensional biological scaffold having a plurality of ordered interconnected pores,
wherein at least three of the plurality of bio-degradable polymer waveguides interpenetrate each other at an aperture of the plurality of apertures, and
wherein the quantity and opening sizes of the plurality of apertures are selected to adjust the quantity and dimensions of the plurality of ordered interconnected pores to facilitate ingress of at least one of biological cells, vascular tissue, or nutrient media.

### Claim 2 (depends on 1)

The method of claim 1, wherein the plurality of ordered interconnected pores occupy a free space of not less than about 40% by volume and not greater than about 99% by volume of the three-dimensional biological scaffold.

### Claim 3 (depends on 1)

The method of claim 1, wherein the plurality of ordered interconnected pores are sized to about 100 μm to 300 μm.

### Claim 4

A method for forming a three-dimensional biological scaffold having a plurality of ordered interconnected pores, the method comprising:
placing a mask having a plurality of apertures between at least one collimated light source and a volume of photo-monomer, in a first position relative to the volume of photo-monomer;
directing a plurality of collimated light beams from the at least one collimated light source through the plurality of apertures into a portion of the volume of photo-monomer, while the mask remains in the first position relative to the volume of photo-monomer, to polymerize the portion to form a plurality of polymer waveguides;
removing photo-monomer of the volume of photo-monomer not polymerized to leave behind the three-dimensional biological scaffold having a plurality of ordered interconnected pores; and
covering surfaces of the plurality of polymer waveguides with at least one coating layer,
wherein at least three of the plurality of polymer waveguides interpenetrate each other at an aperture of the plurality of apertures, and
wherein the quantity and opening sizes of the plurality of apertures are selected to adjust the quantity and dimensions of the plurality of ordered interconnected pores to facilitate ingress of at least one of biological cells, vascular tissue, or nutrient media.

### Claim 5 (depends on 4)

The method of claim 4, wherein the plurality of ordered interconnected pores occupy a free space of not less than about 40% by volume and not greater than about 99% by volume of the three-dimensional biological scaffold.

### Claim 6 (depends on 4)

The method of claim 4, wherein the plurality of ordered interconnected pores are sized to about 100 μm to 300 μm.

### Claim 7 (depends on 4)

The method of claim 4, wherein the at least one coating layer comprises one of metal or ceramic.