# US 8,540,999 B2 · Allergenic proteins and peptides from Japanese cedar pollen

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- Number: US 8,540,999 B2
- Document ID: US8540999B2
- Status: Granted
- Kind: B2
- Application number: 10/931,260
- Filed: 2004-08-30
- Published: 2005-07-14
- Granted: 2013-09-24
- Earliest claimed: 1991-07-12
- Assignees: Merck Patent GmbH
- Applicants: Irwin J. Griffith; Joanne Pollock; Julian F. Bond; Richard D. Garman; Mei-Chang Kuo; Stephen P. Powers; Mark A. Exley; Xian Chen; Ze'ev Shaked
- Inventors: Irwin J. Griffith; Joanne Pollock; Julian F. Bond; Richard D. Garman; Mei-Chang Kuo; Stephen P. Powers; Mark A. Exley; Xian Chen; Ze'ev Shaked
- Web page: https://patentgrep.com/patent/US8540999B2

## CPC

- C07K 14/415: from plants
- C07K 16/16: against material from plants
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention provides nucleic acid sequences coding for the Cryptomeria japonica major pollen allergen Cry j I, Cry j II, Jun s I and Jun v I and fragments or peptides thereof. The present invention also provides purified Cry j I, Cry j II, Jun s I and Jun v I and at least one fragment thereof produced in a host cell transformed with a nucleic acid sequence coding for Cry j I, Cry j II, Jun s I and Jun v I or at least one fragment thereof, and fragments of Cry j I, Cry j II, Jun s I or Jun v I or at least one fragment thereof, and fragments of Cry j I, Cry j II, Jun s I or Jun v I prepared synthetically. Cry j I, Cry j II, Jun s I and Jun v I and fragments thereof are useful for diagnosing, treating, and preventing Japanese cedar pollinosis. The present invention also provides isolated peptides of Cry j I and Cry j II. Peptides within the scope of the invention comprise at least one T cell epitope, or preferably at least two T cell epitopes of Cry j I or Cry j II. The invention also pertains to modified peptides having similar or enhanced therapeutic properties as the corresponding naturally-occurring allergen or portion thereof but having reduced side effects. Methods of treatment or of diagnosis of sensitivity to Japanese cedar pollens in an individual and therapeutic compositions, and multipeptide formulations comprising one or more peptides of the invention are also provided.

## Claims

### Claim 1

An isolated Japanese cedar pollen allergen produced in a host cell transformed with a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, or the coding region thereof, wherein the allergen is recombinantly produced is free of other cedar pollen ailergens and is non-glycosyl.

### Claim 2

An isolated Japanese cedar pollen allergen, comprising the amino acid sequence of SEQ ID NO:2, or the mature portion thereof, wherein the allergen is recombinantly produced is free of other cedar pollen allergens and is non-glycosylated.