# US 8,540,978 B2 · Treatment composition comprising physically disrupted tooth pulp and non-cultured stem cells in a matrix > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,540,978 B2 - Document ID: US8540978B2 - Status: Granted - Kind: B2 - Application number: 12/772,363 - Filed: 2010-05-03 - Published: 2010-08-19 - Granted: 2013-09-24 - Earliest claimed: 2009-01-03 - Applicants: Ray C. Wasielewski - Inventors: Ray C. Wasielewski - Web page: https://patentgrep.com/patent/US8540978B2 ## CPC - A61K 35/32: Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane - A61K 9/0009: {involving or responsive to electricity, magnetism or acoustic waves; Galenical aspects of sonophoresis, iontophoresis, electroporation or electroosmosis} - A61K 38/18: Growth factors; Growth regulators - A61K 38/1875: {Bone morphogenic factor; Osteogenins; Osteogenic factor; Bone-inducing factor} - A61K 38/19: Cytokines; Lymphokines; Interferons - A61K 38/191: {Tumor necrosis factors [TNF], e.g. lymphotoxin [LT], i.e. TNF-beta} - A61K 38/20: Interleukins [IL] - A61L 27/3604: {characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel} - A61L 27/3834: {Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells} - A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like - A61P 19/00: Drugs for skeletal disorders - B33Y 80/00: Products made by additive manufacturing - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - B33Y: ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING ## Abstract A medical implant comprising in components from a tooth and stem cells harvested from at least one tooth. Tooth stem cells may be harvested from the dental pulp of mammalian teeth, such as unerupted third molars in humans. After the stem cells are removed and isolated from the other teeth tissue, the hard tooth may be ground into a base material for the manufacture of a porous matrix into which the tooth stem cells can be added. Additionally, soft tissue from the harvested tooth may be used as a carrier scaffold for soft tissue applications such as meniscal or cartilage repair. ## Claims ### Claim 1 A medical implant comprising: physically disrupted, non-enzyme-digested tooth pulp including a plurality of non-cultured stem cells; and a matrix. ### Claim 2 (depends on 1) The medical implant of claim 1, wherein the physically disrupted, non-enzyme-digested tooth pulp is selected from the group consisting of a diced tooth pulp, a minced tooth pulp, and combinations thereof. ### Claim 3 (depends on 1) The medical implant of claim 1, wherein the physically disrupted, non-enzyme-digested tooth pulp is a sterilized, physically disrupted, non-enzyme-digested tooth pulp, and wherein the sterilized, physically disrupted, non-enzyme-digested tooth pulp includes a plurality of stem cells that are living. ### Claim 4 (depends on 1) The medical implant of claim 1, wherein the plurality of stem cells include pluripotent stem cells. ### Claim 5 (depends on 1) The medical implant of claim 1 wherein the matrix is a biological matrix. ### Claim 6 (depends on 5) The medical implant of claim 5 wherein the biological matrix is chosen from the group consisting essentially of a plurality of mammalian tooth particles, a plurality of bone particles, soft tissue, and combinations thereof. ### Claim 7 (depends on 6) The medical implant of claim 6 wherein the plurality of mammalian tooth particles is a plurality of ground mammalian tooth particles. ### Claim 8 (depends on 7) The medical implant of claim 7, wherein the plurality of ground mammalian tooth particles include dentin, enamel, or mixtures thereof. ### Claim 9 (depends on 7) The medical implant of claim 7, wherein the plurality of ground mammalian tooth particles include particles of tooth derived hydroxyapatite. ### Claim 10 (depends on 7) The medical implant of claim 7, wherein the plurality of mammalian ground tooth particles include one or more endogenous or exogenous growth factors. ### Claim 11 (depends on 10) The medical implant of claim 10, wherein the one or more endogenous or exogenous growth factors are chosen from transforming growth factor beta, insulin like growth factor I, insulin like growth factor II, platelet-derived growth factor, fibroblast growth factor, and bone morphogenetic proteins. ### Claim 12 (depends on 7) The medical implant of claim 7, wherein the plurality of mammalian ground tooth particles have a mean particle size in the range of 5 μm to 100 μm. ### Claim 13 (depends on 7) The medical implant of claim 7, wherein the plurality of ground mammalian tooth particles have a mean particle size in the range of 100 μm to 500 μm. ### Claim 14 (depends on 7) The medical implant of claim 7, wherein the plurality of ground mammalian tooth particles have a mean particle size in the range of 500 μm to 1,000 μm. ### Claim 15 (depends on 5) The medical implant of claim 5 wherein the biological matrix includes an autologous tissue. ### Claim 16 (depends on 1) The medical implant of claim 1 wherein the implant is shaped for implantation into a defect in a tissue. ### Claim 17 (depends on 1) The medical implant of claim 1 wherein the implant is shaped in the shape of at least a portion of a bone. ### Claim 18 A medical implant comprising: physically disrupted non-enzyme-digested tooth pulp including a plurality of stem cells; and a matrix. ### Claim 19 (depends on 18) The medical implant of claim 18 wherein the matrix is chosen from the group consisting essentially of a plurality of mammalian tooth particles, a plurality of bone particles, soft tissue, and combinations thereof. ### Claim 20 A medical implant comprising: physically disrupted, non-enzyme-digested tooth pulp including a plurality of stem cells. ### Claim 21 (depends on 20) The medical implant of claim 20, wherein the physically disrupted, non-enzyme-digested tooth pulp is selected from the group consisting of a diced tooth pulp, a minced tooth pulp, and combinations thereof. ### Claim 22 (depends on 20) The medical implant of claim 20, wherein the physically disrupted, non-enzyme-digested tooth pulp is a sterilized, physically disrupted, non-enzyme-digested tooth pulp, and wherein the sterilized, physically disrupted, non-enzyme-digested tooth pulp includes a plurality of stem cells that are living. ### Claim 23 (depends on 20) The medical implant of claim 20, wherein the plurality of stem cells include pluripotent stem cells. ### Claim 24 (depends on 20) The medical implant of claim 20, wherein the implant is shaped for implantation into a defect in a tissue. ### Claim 25 (depends on 20) The medical implant of claim 20, wherein the implant is shaped in the shape of at least a portion of a bone. ### Claim 26 (depends on 20) The medical implant of claim 20, wherein the stem cells are non-cultured stem cells.