# US 8,540,078 B2 · Liquid separation from adipose tissue > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,540,078 B2 - Document ID: US8540078B2 - Status: Granted - Kind: B2 - Application number: 12/758,119 - Filed: 2010-04-12 - Published: 2010-12-16 - Granted: 2013-09-24 - Earliest claimed: 2009-06-16 - Assignees: Biomet Biologics, LLC - Applicants: Michael D. Leach; Jason Chavarria - Inventors: Michael D. Leach; Jason Chavarria - Web page: https://patentgrep.com/patent/US8540078B2 ## CPC - B01D 21/262: {by using a centrifuge} - B01D 17/0217: {by centrifugal force} - B04B 3/00: Centrifuges with rotary bowls in which solid particles or bodies become separated by centrifugal force and simultaneous sifting or filtering - B04B 5/0442: {with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation} - B04B 11/06: Arrangement of distributors or collectors in centrifuges - A61B 2017/00969: {used for transplantation} - A61M 1/892: {with treatment of the collected fat} - A61M 2202/08: Lipoids - B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28) - B04B: CENTRIFUGES (high-speed drum mills B02C19/11; domestic spin driers D06F; analysing, measuring or monitoring physical or chemical properties of samples during centrifuging, see the relevant subclasses for these procedures, e.g. G01N) - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} ## Abstract Extracting or removing at least a portion of liquid phase from a whole sample using a centrifugal force is disclosed. Centrifugal forces are used to apply pressure to a whole sample and drive a liquid phase through a passage region that can be perforated and/or porous and maintain a drier portion within a separation container. The whole sample can be dried, which includes a remaining sample where excess or a selected amount of liquid is removed. Direct access to the separation container or area can then be made to provide for an efficient withdrawal of the drier material from the separation container. ## Claims ### Claim 1 A fluid reducing system, comprising: a container configured to rotate around an axis of rotation and having an inner wall having an inner surface to contain a whole adipose tissue sample, wherein the inner wall includes a first section where the inner surface is substantially parallel to the axis of rotation and a second section extending from the first section where the inner surface in the second section extends non-parallel to the axis of rotation to define a sump in the second section, wherein the container is positioned within an outer structure to limit access to the container while rotating the container around the axis of rotation; a plurality of throughbores formed through the first section of the inner wall extending from the inner surface to an outer surface in a perforated portion of the inner wall; a porous material covering at least a first plurality of the throughbores; and an access port through the outer structure operable to allow extraction of a drier portion of the whole adipose sample from the second section within the container after drying the whole adipose tissue sample. ### Claim 2 (depends on 1) The fluid reducing system of claim 1, wherein the throughbores have a first average cross section opening greater than a second average cross section opening defined by the porous material. ### Claim 3 (depends on 2) The fluid reducing system of claim 2, wherein the first average cross section opening is at least two times the second average cross section opening, wherein the second average cross section opening is about 0.015 inches or less. ### Claim 4 (depends on 1) The fluid reducing system of claim 1, wherein the porous material is at least one of a woven fabric, a felt, or combinations thereof. ### Claim 5 (depends on 4) The fluid reducing system of claim 4, wherein the plurality of throughbores are a non-limiting structure for removing fluid from the whole adipose tissue sample. ### Claim 6 A fluid reducing system comprising: a container assembly having a first inner container having an inner wall with throughbores formed in the inner wall extending from an inner surface to an outer surface of the inner wall, and a second outer container adjacent to the outer surface of the inner wall, wherein a first wall surface of the second outer container is the same as the outer surface of the inner wall; a top extending over and covering the first inner container and the second outer container; a tube extending through the top and to a central depression in the first inner container; and a felt material positioned in the first inner container to cover the throughbores; wherein a first whole sample is positioned in the first inner container and the tube is operable to be used to withdraw a drier material from the central depression; wherein the central depression is a sump defined by the inner surface of the inner wall; wherein a fluid from the whole sample must pass through the felt material first and then the throughbores to enter the second outer container. ### Claim 7 (depends on 6) The fluid reducing system of claim 6, wherein the throughbores have a first average cross section opening greater than a second average cross section opening defined by the felt material. ### Claim 8 (depends on 7) The fluid reducing system of claim 7, wherein the first average cross section opening is at least two times the second average cross section opening, wherein the second average cross section opening is about 0.015 inches or less. ### Claim 9 (depends on 6) The fluid reducing system of claim 6, wherein the second outer container has a second outer container volume; wherein an outer wall of the second outer container is operable to be positioned relative to the first inner container to define the second outer container volume; wherein the volume of the second outer container limits the volume of liquid removed from the first whole sample positioned in the first inner container. ### Claim 10 (depends on 6) The fluid reducing system of claim 6, further comprising: an outer housing including a member operable with the tube to access the central depression in the first inner container from a position exterior to the outer housing. ### Claim 11 A fluid reducing system comprising: a container assembly having a first inner container having an inner wall with throughbores formed in the inner wall extending from an inner surface to an outer surface of the inner wall, and a second outer container adjacent to the outer surface of the inner wall, wherein a first wall surface of the second outer container is the same as the outer surface of the inner wall; a top extending over and covering the first inner container and the second outer container; a tube extending through the top and to a central depression in the first inner container; and a woven material positioned in the first inner container to cover the throughbores; wherein a first whole sample is positioned in the first inner container and the tube is operable to be used to withdraw a drier material from the central depression; wherein the central depression is a sump defined by the inner surface of the inner wall; wherein a fluid from the first whole sample must pass through the woven material first and then the throughbores to enter the second outer container. ### Claim 12 A fluid reducing system, comprising: a separation container having: an exterior wall that is substantially parallel about a central axis of the separation container; an annular collection basin formed between an inner wall spaced towards the central axis from the exterior wall; a passage formed in a perforated portion of the inner wall through the inner wall from an inner surface to an outer surface of the inner wall; and a porous filter material covering the perforated portion of the inner wall; wherein the perforated portion of the inner wall is substantially parallel to the central axis of the separation container; and an exterior housing that houses the separation container; wherein the exterior wall is non-parallel to the central axis of the separation container at an angled portion of the separation container separate from the perforated portion and a portion of the inner wall is parallel to the exterior wall away from the perforated portion of the inner wall that is substantially parallel to the central axis of the separation container. ### Claim 13 (depends on 12) The fluid reducing system of claim 12, wherein the passage of the perforated portion of the inner wall includes a plurality of throughbores formed through the inner wall having a first average cross-section opening greater than a second average cross-section opening defined by the porous filter material. ### Claim 14 (depends on 12) The fluid reducing system of claim 12, further comprising, an inner lid fit over the exterior wall to seal the separation container including the annular collection basin; and a withdrawal tube extending through the inner lid to a central depression in the separation container. ### Claim 15 (depends on 14) The fluid reducing system of claim 14, the withdrawal tube extends into both the exterior housing and the separation container. ### Claim 16 (depends on 12) The fluid reducing system of claim 12, wherein the porous filter material is positioned either adjacent the inner surface of the perforated portion or adjacent the outer surface of the perforated portion.