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US 8,535,889 B2Granted
Digital analyte analysis
AssigneeRaindance Technologies, Inc.
InventorsJonathan William Larson, Qun Zhong, Darren Roy Link
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Abstract
The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
Claims14
A method for analyzing a target nucleic acid, the method comprising the steps of:
- forming droplets containing a single target nucleic acid and one or more amplification reagents;
- amplifying the target in the droplets;
- distinguishing droplets containing amplicon from the target and amplicon from a variant of the target generated by polymerase error by using two differently labeled-hybridization probes, one hybridizing to the target and one hybridizing to a specific variant of the target;
- and
- analyzing target amplicons.
2 more elements
Dependencies are inferred from the claim text.