# US 8,535,684 B2 · Methods of inhibiting HIV infectivity

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- Number: US 8,535,684 B2
- Document ID: US8535684B2
- Status: Granted
- Kind: B2
- Application number: 12/652,877
- Filed: 2010-01-06
- Published: 2010-07-22
- Granted: 2013-09-17
- Earliest claimed: 2009-01-06
- Assignees: Functional Genetics, Inc.
- Applicants: Michael S. Kinch; Michael Goldblatt; Wu-Bo Li; Douty Bamba; Shaojing Chang; Huosheng Chen; Zenbework Fesseha; Manu Kohli; Hanwen Mao; Heather Thi Thu Ung-Medoff; Ke Weng
- Inventors: Michael S. Kinch; Michael Goldblatt; Wu-Bo Li; Douty Bamba; Shaojing Chang; Huosheng Chen; Zenbework Fesseha; Manu Kohli; Hanwen Mao; Heather Thi Thu Ung-Medoff; Ke Weng
- Web page: https://patentgrep.com/patent/US8535684B2

## CPC

- C07K 16/28: against receptors, cell surface antigens or cell surface determinants
- A61P 31/16: for influenza or rhinoviruses
- A61P 31/18: for HIV
- C12N 15/1082: {Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.

## Claims

### Claim 1

A method of inhibiting the infectivity of HIV in mammalian cells, comprising:
selecting mammalian cells in need of inhibition of HIV infectivity and contacting said cells with an effective amount of an antibody that binds preferentially to Robo1 protein.

### Claim 2 (depends on 1)

The method of claim 1, wherein said selecting is accomplished by selecting a mammal in need of reducing the infectivity of HIV and said contacting is achieved by administering said antibody to said mammal.

### Claim 3 (depends on 1)

The method of claim 1, wherein said method comprises administering to said mammal a Robo 1 protein which induces said mammal to produce antibodies which bind preferentially to Robo1 protein, thereby contacting cells of said mammal with said antibodies produced.

### Claim 4 (depends on 2)

The method of claim 2, wherein said antibody is a monoclonal antibody.