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US 8,530,171 B2Granted
High level expression of recombinant toxin proteins
AssigneePfenex Inc.
InventorsDiane M. Retallack, Lawrence Chew, Hongfan Jin, Henry W. Talbot
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Abstract
The present invention relates to the field of recombinant toxin protein production in bacterial hosts. In particular, the present invention relates to production processes for obtaining high levels of a recombinant CRM197, Diphtheria Toxin, Pertussis Toxin, Tetanus Toxoid Fragment C, Cholera Toxin B, Cholera holotoxin, and Pseudomonas Exotoxin A, from a bacterial host.
Claims36
A method for producing a recombinant toxin protein in a Pseudomonad host cell, said method comprising:
- ligating into an expression vector a nucleotide sequence encoding the toxin protein;
- transforming the Pseudomonad host cell with the expression vector;
- and
- culturing the transformed Pseudomonad host cell in a culture media suitable for the expression of the recombinant toxin protein;
- wherein the recombinant toxin protein is CRM 197, and wherein the recombinant protein is produced at a yield of soluble or active CRM197 protein of about 0.2 grams per liter to about 12 grams per liter.
2 more elements
Dependencies are inferred from the claim text.