Back to results
US 8,529,744 B2Granted
Enrichment of nucleic acid targets
AssigneeBoreal Genomics, Inc.
InventorsAndrea Marziali, Nitin Sood
Life of this patent
Estimating the term…
Abstract
Methods and apparatus providing improved fidelity and specificity when separating nucleic acids from a sample, but without need for amplification. In particular, using the disclosed methods, it is possible to isolate a variant nucleic acid (i.e., a mutation) from a non-target nucleic acid (i.e., a wild-type) when the variant is present in the original sample at a much lower concentration than the non-target, e.g., 1:10,000, without substantial loss of the variant.
Claims26
A method for enriching a sample, the method comprising the steps of:
- obtaining a sample comprising a non-target nucleic acid and a target nucleic acid, wherein the target nucleic acid and the non-target nucleic acid differ by only a single base at a position of interest but do not differ in length, and the target to non-target ratio in the sample is X;
- and
- enriching for the target nucleic acid by applying a time-varying driving field to the sample in the presence of a separation medium,
- wherein:
- (a) after enrichment the ratio of target nucleic acid to non-target nucleic acid is Y, wherein Y is at least 10 times greater than X, and
- no new molecules are generated in the sample during said enriching step.
3 more elements
Dependencies are inferred from the claim text.