Accurate identification of organisms based on individual information content

A method for identification of an organism, comprising the steps of:

• a) aligning a first polynucleotide sequence known to exist in a first organism with at least one additional related polynucleotide sequence from at least one different organism, said at least one additional related polynucleotide sequence being different from one another and from said first polynucleotide sequence;
• b) searching for a window with low average information content on said aligned sequences;
• c) selecting from said first polynucleotide sequence at least one segment with low individual information content or a portion thereof as an oligonucleotide for identification of said first organism;
• and
• d) hybridizing said oligonucleotides with at least one DNA molecule obtained from said organism,
• wherein the average information content is determined by calculating and comparing the values of Rsequence for every equal-length window on said aligned sequences.

A method for identification of a type strain, comprising the steps of:

• a) aligning a first polynucleotide sequence known to exist in a first type strain with at least one additional polynucleotide sequence from at least one different type strain, wherein said at least one additional polynucleotide sequence is different from one another and from said first polynucleotide sequence, and said first type strain and said at least one different type strain belong to the same species;
• b) searching for a window with low average information content on said aligned sequences;
• and
• c) selecting from said first polynucleotide sequence at least one segment with low individual information content or a portion thereof as an oligonucleotide for identification of said first type strain.

A method for identification of an organism, comprising the steps of:

• a) aligning a first polynucleotide sequence known to exist in a first organism with at least one additional related polynucleotide sequence from at least one different organism, said at least one additional polynucleotide sequence being different from one another and from said first polynucleotide sequence;
• b) searching for a window with low average information content on said aligned sequences, wherein the average information content is determined by calculating and comparing the values of Rsequence for every equal-length segment in the aligned sequences;
• and
• c) selecting from said first polynucleotide sequence at least one segment with low individual information content or a portion thereof as an oligonucleotide for identification of said first organism.