Method and apparatus of measuring relative phase of bio-cells
AssigneeGwangju Institute of Science and Technology
InventorsIn Hee Shin, Sang Mo Shin
Life of this patent
Estimating the term…
Abstract
A method of measuring a relative phase of a bio-cell using a digital image sensor, comprising the steps of firstly filtering a light emitted from a light source, using a first polarizer and a first wave plate, which are arranged in order in a optical path, exposing a bio-cell to the firstly filtered light, secondly filtering the light passing through the bio-cell, using a second wave plate and a second polarizer, which are arranged in order in the optical path, and sensing an intensity of the secondly filtered light, by each of pixels of the image sensor, wherein, as conditions of the second filtering are varied, optical properties of the bio-cell are calculated using the intensity of the light in a pixel-wise manner.
Claims8
A method of measuring a relative phase of a bio-cell using a digital image sensor, the method comprising the steps of:
- firstly filtering a light emitted from a light source, using a first polarizer and a first wave plate, which are arranged in order on an optical path;
- exposing a bio-cell to the firstly filtered light;
- secondly filtering the light passing through the bio-cell, using a second wave plate and a second polarizer, which are arranged in order on the optical path;
- and
- sensing an intensity of the secondly filtered light, by each of pixels of an image sensor,
- wherein, as conditions of the second filtering are varied, optical properties of the bio-cell are calculated using the intensity of the light in a pixel-wise manner,
- wherein the first wave plate and the second wave plate are provided by using a quarter-wavelength plate,
- wherein the first wave plate and the second wave plate are positioned at 45° and 135° respectively to a polarization axis defined by the first polarizer, and
- wherein a Jones matrix of the optical properties calculated in a pixel-wise manner is as follows:
- [
- E
- x
- E
- y
- ]
- =
- [
- cos
-
-
- β
- -
- sin
-
-
- β
- sin
-
-
- β
-
- cos
-
-
- β
- ]
-
- [
- 1
- 0
- 0
- 0
- ]
-
- [
- cos
-
-
- β
- sin
-
-
- β
- -
- sin
-
-
- β
- cos
-
-
- β
- ]
- [
-
- cos
-
- (
- 135
-
- °
- )
- -
- sin
-
- (
- 135
-
- °
- )
- sin
-
- (
- 135
-
- °
- )
- cos
-
- (
- 135
-
- °
- )
- ]
-
- [
-
- ⅇ
- ⅈ
-
- π
- 4
- 0
- 0
- ⅇ
- -
- ⅈ
-
- π
- 4
- ]
-
- [
- cos
-
- (
- 135
-
- °
- )
- sin
-
- (
- 135
-
- °
- )
- -
- sin
-
- (
- 135
-
- °
- )
- cos
-
- (
- 135
-
- °
- )
- ]
- ×
- [
- ⅇ
- ⅈ
-
- δ
- 2
- 0
- 0
- ⅇ
- ⅈ
-
- δ
- 2
- ]
-
- [
- cos
-
-
- 45
- -
- sin
-
-
- 45
- sin
-
-
- 45
- cos
-
-
- 45
- ]
-
- [
-
- ⅇ
- ⅈ
-
- π
- 4
- 0
- 0
- ⅇ
- -
- ⅈ
-
- π
- 4
- ]
-
- [
- cos
-
-
- 45
- sin
-
-
- 45
- -
- sin
-
-
- 45
- cos
-
-
- 45
- ]
-
- [
- 0
- 1
- ]
-
- E
- 0
-
- ⅇ
- ⅈ
-
-
- ω
-
-
- t
- whereby Ex and Ey are intensities of the light respectively to x axis and y axis, β is a rotating angle of the second polarizer, δ is a phase delay by the bio-cell, E0 is an amplitude of an initial light, ω is a frequency of the initial light.
An apparatus of measuring a relative phase of a bio-cell comprising:
- a light source member having a light source emitting light;
- a first polarizer for polarizing the light from the light source to a specific direction of polarization state;
- a first wave plate for affecting the polarization direction of the light passing through the first polarizer;
- a stage plate for locating the bio-cell on an optical path of the light passing through the first wave plate;
- a second wave plate for affecting the polarization direction of the light passing though the stage plate;
- a second polarizer for polarizing the light passing through the second wave plate to another specific direction of polarization state;
- and
- an image reading member having an image sensor detecting intensities of the light passing through the second polarizer,
- wherein the first wave plate and the second wave plate are provided by using a quarter-wave length plate,
- wherein the first wave plate and the second wave plate are positioned at 45° and 135° respectively to a polarization axis defined by the first polarizer, and
- wherein a Jones matrix of the optical properties calculated in a pixel-wise manner is as follows:
- [
- E
- x
- E
- y
- ]
- =
- [
- cos
-
-
- β
- -
- sin
-
-
- β
- sin
-
-
- β
-
- cos
-
-
- β
- ]
-
- [
- 1
- 0
- 0
- 0
- ]
-
- [
- cos
-
-
- β
- sin
-
-
- β
- -
- sin
-
-
- β
- cos
-
-
- β
- ]
- [
-
- cos
-
- (
- 135
-
- °
- )
- -
- sin
-
- (
- 135
-
- °
- )
- sin
-
- (
- 135
-
- °
- )
- cos
-
- (
- 135
-
- °
- )
- ]
-
- [
-
- ⅇ
- ⅈ
-
- π
- 4
- 0
- 0
- ⅇ
- -
- ⅈ
-
- π
- 4
- ]
-
- [
- cos
-
- (
- 135
-
- °
- )
- sin
-
- (
- 135
-
- °
- )
- -
- sin
-
- (
- 135
-
- °
- )
- cos
-
- (
- 135
-
- °
- )
- ]
- ×
- [
- ⅇ
- ⅈ
-
- δ
- 2
- 0
- 0
- ⅇ
- ⅈ
-
- δ
- 2
- ]
-
- [
- cos
-
-
- 45
- -
- sin
-
-
- 45
- sin
-
-
- 45
- cos
-
-
- 45
- ]
-
- [
-
- ⅇ
- ⅈ
-
- π
- 4
- 0
- 0
- ⅇ
- -
- ⅈ
-
- π
- 4
- ]
-
- [
- cos
-
-
- 45
- sin
-
-
- 45
- -
- sin
-
-
- 45
- cos
-
-
- 45
- ]
-
- [
- 0
- 1
- ]
-
- E
- 0
-
- ⅇ
- ⅈ
-
-
- ω
-
-
- t
- whereby Ex and Ey are intensities of the light respectively to x axis and y axis, β is a rotating angle of the second polarizer, δ is a phase delay by the bio-cell, E0 is an amplitude of an initial light, ω is a frequency of the initial light.
Dependencies are inferred from the claim text.