Body fluid detection method using surface enhanced Raman spectroscopy

A body fluid detection method using surface enhanced Raman spectroscopy SERS comprising:

• a) treating body fluid sample into aqueous phase in an upper layer and solid phase in a bottom layer;
• b) dividing the upper aqueous phase equally into two parts and electrophoresing appropriate amount of each part simultaneously by membrane electrophoresis;
• c) staining one of the parts to compare with the other unstained part's position in membrane electrophoresis substrates;
• d) cutting off the unstained samples together with the said substrates at the same position when fixing the unstained part's position in the membrane electrophoresis substrates;
• e) bringing the unstained part to contacting with a proper glacial acetic acid at room temperature and mixing them to form limpid colloid, then adding enhancing substrates for Raman spectroscopy, mixing them up and keeping incubating and stirring at 25-65 Celsius;
• stopping stirring but continuing to incubate for 10-40 min when solid impurities precipitating, then taking said mixture of body fluid samples and said enhancing substrates for Raman spectroscopy which is the upper layer formed while standing to be tested through surface enhanced Raman spectroscopy:
• f) setting up SERS database by repetition involving different body fluid samples following the same procedures as described above;
• g) carrying out cluster analysis of SERS database using Principal Component Analysis PCA method and building a SERS analytical model to obtain a scatterplot corresponding to different body fluid samples.