High lycopene content tomato plants and markers for use in breeding for same
AssigneeThe Penn State Research Foundation
InventorMajid R. Foolad
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Abstract
Solanum pimpinellifolium plants which are exceptionally high in fruit lycopene content (˜300-390 μg lycopene/g fresh fruit) are disclosed herein, which may be used in plant breeding to make the tomato plants of the present invention. The invention also discloses genetic markers, which are associated with the trait which may be used in marker-assisted selection for development of plants, lines, or varieties with the trait.
Claims12
A high lycopene content of greater than 100 μg/g fresh tomato tissue Solanum lycopersicum plant or part thereof, having within its genome a QTL associated with high lycopene content on chromosome 7 and a QTL associated with high lycopene content on chromosome 12, of Solanum pimpinellofolium LA2093 having ATCC Accession No. PTA-120390, wherein said QTL is not in said Solanum lycopersicum natural genetic background, and wherein the location of said QTL on chromosome 7 is indicated by a genomic region bound by genetic markers cTOS19O5 and cLEN14F9 and said QTL on chromosome 12 is indicated by a genomic region bound by genetic markers OH275 and T0800.
A method of producing a high lycopene Solanum lycopersicum tomato plant, having a lycopene content of greater than 100 μg/g fresh tomato tissue comprising:
- providing a high lycopene Solanum pimpinellofolium LA2093 having ATCC Accession No. PTA-120390 donor plant;
- transferring a nucleic acid from said donor plant to one or more Solanum lycopersicum tomato plants, wherein the transfer results in the introduction of genomic materials from the donor plant in the corresponding region of the genome of one or more recipient plants;
- and
- selecting from amongst said recipient tomato plants a plant that comprises within its genome a QTL for high lycopene content, from Solanum pimpinellofolium LA2093 on chromosomes 7 and 12, wherein said selection comprises detecting on chromosomes 7 and 12 a genetic marker linked to said QTL for high lycopene content, wherein the location of said QTL on chromosomes 7 and 12 of said plant is indicated by a genomic region bounded by and comprising the genetic markers cTOS 19O5 and cLEN14F9, and OH275 and T0800, respectively.
Dependencies are inferred from the claim text.