Method for deblocking of labeled oligonucleotides
AssigneeLife Technologies Corporation
InventorsGulilat Gebeyehu, Richard Pires
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Abstract
The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.
Claims20
A process for deblocking substantially a blocked, detectably labeled oligonucleotide comprising contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound in the presence of one or more carbohydrates and/or sugars under conditions that result in the deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.
A process for deblocking substantially a blocked, detectably labeled oligonucleotide comprising contacting the blocked detectably labeled oligonucleotide with an effective amount of ammonia saturated with water vapor at about 80 psi, 95° C., for about 45 min, thereby giving the substantially deblocked oligonucleotide.
A composition comprising a blocked, detectably labeled oligonucleotide and an effective amount of a nucleophilic amino compound sufficient to deblock substantially said oligonucleotide and one or more carbohydrates and/or sugars.
Dependencies are inferred from the claim text.