Nucleic acids encoding SOX10 promoter and methods to isolate SOX10 expressing cells
AssigneeCalifornia Institute of Technology
InventorsTatjana Sauka-Spengler, Sonja J. McKeown, Paola Betancur, Marianne Bronner
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Abstract
DNA enhancer sequences are provided for use in constructs to identify early stage embryonic cells. The enhancer sequences can be used in parallel with short-hairpin RNA in a vector construct for endogenously regulated gene knockdowns. The disclosed enhancer sequences can be used to isolate a selected population of early stage embryonic cells.
Claims9
An isolated nucleic acid sequence comprising SEQ ID NO:
- 1.
A nucleic acid vector for down-regulating gene expression comprising:
- a short-hairpin RNA sequence under transcriptional control of at least one enhancer sequence having the sequence as set forth in SEQ ID NO:
- 1.
An expression vector comprising the sequence of SEQ ID NO:
- 1 and a nucleic acid sequence encoding a reporter protein.
A method of isolating mammalian cells expressing SOX10, the method comprising:
- introducing to a population of isolated mammalian cells an expression vector comprising the sequence of SEQ 1D NO:
- 1 and a nucleic acid sequence encoding a reporter protein;
- and
- identifying introduced cells that produce the reporter protein.
Dependencies are inferred from the claim text.