Method for detecting a target molecule in a biological sample
AssigneeThe Cleveland Clinic Foundation
InventorDavid J. Kennedy
Life of this patent
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Abstract
A method is provided for detecting a target molecule in a biological sample. One step of the method includes immobilizing the biological sample on a membrane. Next, the membrane-bound biological sample is contacted with at least one detection moiety. The membrane-bound biological sample is then separately mated with a substrate and the target molecule detected. At least one step of the method is performed under positive pressure or a vacuum.
Claims14
A method for detecting a target molecule in a biological sample, said method comprising the steps of:
- (a) immobilizing the biological sample on a membrane;
- (b) contacting the membrane-bound biological sample with at least one detection moiety;
- (c) separately mating the membrane-bound biological sample with a substrate;
- and
- (d) detecting the target molecule with the at least one detection moiety;
- wherein at least one step of said method is performed under positive pressure or a vacuum.
An immunohistochemical method for detecting a target protein in a biological sample, said method comprising the steps of:
- (a) immobilizing the biological sample on a membrane;
- (b) contacting the membrane-bound biological sample with at least one detection moiety;
- (c) separately mating the membrane-bound biological sample with a substrate;
- and
- (d) detecting the target protein with the at least one detection moiety;
- wherein at least one step of said method is performed under positive pressure or a vacuum.
Dependencies are inferred from the claim text.