Animal protein free media for cultivation of cells

A method of cultivating mammalian surface-dependent cells to obtain a confluent cell culture, comprising:

• providing an animal protein-free medium comprising soy hydrolysate at a concentration of about 0.05% (w/v) to about 1% (w/v) and yeast hydrolysate at a concentration of about 0.05% (w/v) to about 0.3% (w/v);
• growing the surface-dependent cells in the medium;
• and
• passaging and sub-cultivating the cells grown in the medium in order to obtain a confluent cell culture;
• wherein the cells are infected with an orthomyxovirus and are selected from the grouping consisting of BSC-1 cells, CV-1 cells, COS cells, VERO cells, MDBK cells, MDCK cells, MDOK cells, BHK-21 cells, WI-38 cells, and MRC-5 cells.