Low oxygen tension and BFGF generates a multipotent stem cell from a fibroblast in vitro
AssigneeHistogen, Inc.
InventorsGail K. Naughton, Frank Ziegler, Mark Baumgartner, Kyle Nickey
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Abstract
The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
Claims7
A method of generating a multipotent stem cell comprising culturing fibroblast cells under conditions to induce de-differentiation of the fibroblast cells, wherein the conditions are from about 1-5% oxygen on a microcarrier bead surface in a suitable growth medium containing basic FGF (bFGF), thereby generating cells that express genes characteristic of multipotent stem cells.
Dependencies are inferred from the claim text.