Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases
AssigneeE. I. Du Pont de Nemours and Company
InventorsNarendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Abstract
Methods for increasing C18 to C20 elongation conversion efficiency and/or Δ4 desaturation conversion efficiency in long-chain polyunsaturated fatty acid [“LC-PUFA”]-producing recombinant oleaginous microbial host cells are provided herein, based on over-expression of acyl-CoA:lysophospholipid acyltransferases [“LPLATs”] (e.g., Ale1, LPAAT, LPCAT). Production host cells and oils produced by the methods of the invention are also claimed.
Claims8
A recombinant oleaginous Yarrowia lipolytica host cell for improved production of at least one long-chain polyunsaturated fatty acid, said host cell comprising at least one isolated polynucleotide encoding a polypeptide having at least acyl-CoA:lysophospholipid acyltransferase activity, wherein the polypeptide comprises SEQ ID NO:19 and SEQ ID NO:20, wherein the polypeptide has at least 90% amino acid identity, based on the Clustal W method of alignment, when compared to SEQ ID NO:17;
- wherein the isolated polynucleotide is operably linked to at least one regulatory sequence, and wherein the total fatty acids of the host cell comprise at least 51% by weight eicosapentaenoic acid;
- and further wherein the host cell has an increase in C18 to C20 elongation conversion efficiency compared to a control host cell.
Dependencies are inferred from the claim text.