Bacterium producing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity and a method for manufacturing the product

A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity, comprising:

• A) cultivating a bacterium transformed with a DNA fragment comprising a gene coding for a protein having 2-oxo-glutarate-dependent enzymatic activity, wherein said bacterium has been modified to attenuate the expression of a gene coding for oxoglutarate dehydrogenase, in a culture medium containing a substrate of the reaction;
• and wherein said protein has L-isoleucine dioxygenase activity and catalyzes a reaction coupled with the formation of succinate from 2-oxoglutarate;
• and
• B) isolating the product, wherein said product is (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof.